FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327747
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83422.5452559443717258No Hit
GAGTATACTCTCACCATCACCAATCTACAACCTGAAGACTTTGCAACTTA12330.37620481652005966No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11910.36339005391353696No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG9080.2770429630172053No Hit
GTCTGGGACAGAGTATACTCTCACCATCACCAATCTACAACCTGAAGACT8130.24805719045483254No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7800.2379884484068504No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7360.2245634590095409No Hit
GAGTATACTCTGTCCCAGACCCACTGCCACTGAATCGTGCTGGGACCCCA7260.22151232505560692No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6910.210833356216838No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6900.2105282428214446No Hit
GTCCCAGACCCACTGCCACTGAATCGTGCTGGGACCCCACTTTGCAAATT6590.20106972756424923No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT5600.17086350142030285No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5590.17055838802490947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5020.15316692448748578No Hit
GGGTCACACTACCCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAAATCA4840.1476748833704046No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4770.14553908960265083No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG4760.14523397620725742No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACGCTGCATCCAATTTGCAA4750.14492886281186404No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4580.13974193509017627No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4450.13577546095006207No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAA4420.1348601207638819No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4420.1348601207638819No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG4410.1345550073684885No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4370.13333455378691492No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA4300.13119876001916111No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA4280.13058853322837433No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG4250.12967319304219413No Hit
GATCTACGCTGCATCCAATTTGCAAAGTGGGGTCCCAGCACGATTCAGTG4190.12784251266983376No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT4150.12662205908826016No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG4050.12357092513432617No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTG3970.12113001797117899No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA3950.12051979118039219No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG3880.1183839974126384No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3630.11075616252780346No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT3620.11045104913241005No Hit
ACACTACCCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA3590.10953570894622985No Hit
GGGTAGTGTGACCCTGTTGACAGCAGTAAGTTGCAAAGTCTTCAGGTTGT3530.10770502857386949No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3530.10770502857386949No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG3520.10739991517847607No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT3460.1055692348061157No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT3450.1052641214107223No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC3300.10068742047982132No Hit
CCTGAAGACTTTGCAACTTACTGCTGTCAACAGGGTCACACTACCCCTCG3290.10038230708442793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)