FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327747
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47501.4492886281186401No Hit
GAGTATACTCTCACCATCACCAATCTACAACCTGAAGACTTTGCAACTTA9510.2901628390191215No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9390.28650147827440064No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG7930.2419549225469646No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7560.23066572691740886No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6690.20412086151818323No Hit
GAGTATACTCTGTCCCAGACCCACTGCCACTGAATCGTGCTGGGACCCCA6280.1916112123070539No Hit
GTCTGGGACAGAGTATACTCTCACCATCACCAATCTACAACCTGAAGACT6210.18947541853930014No Hit
GTCCCAGACCCACTGCCACTGAATCGTGCTGGGACCCCACTTTGCAAATT5790.1766606559327774No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5750.17544020235120383No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5570.16994816123412265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5280.16109987276771412No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4220.12875785285601393No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG4050.12357092513432617No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3880.1183839974126384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG3820.11655331704027802No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT3690.11258684290016384No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3690.11258684290016384No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACGCTGCATCCAATTTGCAA3650.11136638931859026No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT3640.11106127592319685No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA3530.10770502857386949No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTG3490.10648457499229588No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG3460.1055692348061157No Hit
GGGTCACACTACCCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAAATCA3460.1055692348061157No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG3450.1052641214107223No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3450.1052641214107223No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT3420.1043487812245421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)