FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256599
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113704.431038312698023No Hit
ATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCT16710.6512106438450657No Hit
ATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATA10840.4224490352651413No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10660.4154341988861999No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA7800.30397624308746335No Hit
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATG7590.2957922673120316No Hit
GGGTAACCCCGGCAGATTGCGCGATCACGCGCGTCCCCCGAAAGGGAATC6220.24240156820564382No Hit
TCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACCGCGC5910.23032046110857798No Hit
ACCGAGTACCGTCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCA5870.22876160857992434No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5450.21239365702906088No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATT5450.21239365702906088No Hit
GTGTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACC5450.21239365702906088No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5430.2116142307647341No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT5220.2034302549893024No Hit
GTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATTCCCGGTCCGTCCCC4810.18745201657060237No Hit
ACCCACACCCGGCCATCTGGGCGAGCGCCATGCCCCGATGAGTAGGAGGG4700.1831651721168048No Hit
CCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATAAATAAGCGG4340.1691354993589219No Hit
GGTGTGGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC4310.16796635996243167No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTC4260.16601779430161456No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4180.16290008924430727No Hit
TGGGTAAGCCGATCCTAAGGGACGGGGTAACCCCGGCAGATTGCGCGATC4040.15744410539401946No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC3950.15393668720454873No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA3870.15081898214724143No Hit
GACCGGGCGTGGACGGTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGG3820.14887041648642432No Hit
TTCCCAAACAACCCGACTCGTTGACAGCGCCTCGTGATGCGACAGGGTCC3750.14614242456128043No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3650.1422452932396463Clontech Universal Primer Mix Long (96% over 26bp)
GGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGTGCAAGTGCCGTTCA3630.14146586697531946No Hit
GTCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG3510.1367893093893585No Hit
TTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACC3480.13562016999286824No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG3450.134451030596378No Hit
GGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCG3440.1340613174642146No Hit
ATGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATAA3380.1317230386712341No Hit
ACCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATAAATAAGCG3380.1317230386712341No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC3310.1289950467460902No Hit
TCCTAACGTCGCCGTCAACCGCCACATCCCGGCTCGGGAAATCTTAACCC3270.12743619421743654No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT3230.1258773416887829No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT3200.12470820229229264No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC3080.12003164470633165No Hit
CAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGA3010.11730365278118776No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAA2970.11574480025253411No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT2960.1153550871203707No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGA2940.11457566085604387No Hit
GTTCGGGGCCGGGACCCCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCC2930.11418594772388045No Hit
GGCGTGGACGGTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGC2900.11301680832739021No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2880.11223738206306338No Hit
GCGCTGAAGCGCGCGACCCACACCCGGCCATCTGGGCGAGCGCCATGCCC2850.11106824266657314No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT2840.11067852953440971No Hit
GTGCTCTTCCGACCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGT2820.1098991032700829No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT2730.10639168508061216No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT2730.10639168508061216No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT2690.10483283255195851No Hit
GTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAA2680.10444311941979509No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTT2660.10366369315546826No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC2640.10288426689114144No Hit
GCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCC2610.1017151274946512No Hit
GTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACCGT2600.10132541436248778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)