FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002146952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002146952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387127
Sequences flagged as poor quality0
Sequence length20-100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313688.102767308919296No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22280.5755217280117377Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21640.5589896855553863Clontech Universal Primer Mix Long (96% over 26bp)
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC21570.5571814934117227No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18530.478654291744053No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA15670.4047767270172321No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG15430.3985772110961003No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA14840.3833367344566512Clontech Universal Primer Mix Long (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT14490.3742957737383339No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC13960.3606051760791678No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13680.353372407504514No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT13190.34071506249886985No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT12400.32030832259181097No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT11950.3086842302396888No Hit
ATGGGAAGCAGTGGTATCAACGC11850.30610109860588386No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT11700.30222640115517646No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA11550.29835170370446906No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA11140.28776086400586887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT10960.28311122706502No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG10510.27148713471289787No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTT10120.26141292134105865No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT10050.2596047291973952No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTT10010.2585714765438732No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9760.2521136474593609No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9640.249013889498795No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT9420.24333099990442414No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG9070.23429003918610689Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG8580.22163269418046275Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC8560.22111606785370175Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA8420.21749968356637484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT8200.21181679397200404No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTT8050.2079420965212966No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC7990.20639221754101367Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC7770.20070932794664284No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7740.19993438845650136Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG7620.19683463049593544No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC7430.19192668039170607No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG7140.1844355986536718Clontech Universal Primer Mix Long (96% over 25bp)
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT6940.17926933538606193No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6690.17281150630154962Clontech SMART CDS Primer II A (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG6470.16712861670717877Clontech Universal Primer Mix Long (96% over 25bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT6200.16015416129590548No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGA6190.15989584813252497Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA6020.1555045243550566Clontech SMART CDS Primer II A (96% over 26bp)
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA6010.15524621119167611No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6010.15524621119167611Clontech Universal Primer Mix Long (96% over 26bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC5700.14723850312688083No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTT5690.14698018996350035No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.14646356363673937No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA5670.14646356363673937No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG5650.14594693730997838No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT5560.14362211883955395No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT5550.14336380567617346No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG5530.14284717934941246No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA5460.14103898720574903No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTT5420.14000573455222703No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5400.13948910822546606No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTT5390.13923079506208558No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA5370.13871416873532458No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTT5360.1384558555719441No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5340.13793922924518312No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG5110.13199802648743178No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTT5040.13018983434376832No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5040.13018983434376832No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4950.12786501587334387No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT4900.12657345005644144No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC4810.124248631586017No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4790.12373200525925601Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTT4770.12321537893249501No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC4760.12295706576911451No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4540.1172741761747437No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4470.11546598403108024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG4440.11469104454093877Clontech Universal Primer Mix Long (96% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4340.11210791290713383Clontech Universal Primer Mix Long (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA4170.10771658912966547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4130.10668333647614349No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG4120.10642502331276299No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.10565008382262152No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA4060.10487514433248003No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCT3960.1022920126986751No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTT3930.10151707320853363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)