FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241325
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.6339997928105252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.3663109914016368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.2527711592251114No Hit
CTCCTTAGCTGATGCCGGCTTCCATGGCCACAGCCCGACTGTCTATATGGGACAACACCTTTTATGGTTGTCATC6020.24945612762871647No Hit
GTCTATATGGGACAACACCTTTTATGGTTGTCATCGCCCAGCGATTTGTAGCCCTAAGTCAACGTTTGGTTCATC4140.17155288511343625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4060.16823785351704135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3940.163265306122449Clontech Universal Primer Mix Long (96% over 26bp)
GAGTATGACTATCGGCATATTAACGATACCAGGTCCGACTACGTCCATTTTCAGGGCCGCCCCATTCGGTCGGTG3890.16119341137470217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.15787837977830727No Hit
TACCAGGTCCGACTACGTCCATTTTCAGGGCCGCCCCATTCGGTCGGTGAGCTGTTACGCACTCCTTAGCTGATG3140.13011499015849995No Hit
GTTACGCACTCCTTAGCTGATGCCGGCTTCCATGGCCACAGCCCGACTGTCTATATGGGACAACACCTTTTATGG3120.12928623225940122No Hit
CCTTAGCTGATGCCGGCTTCCATGGCCACAGCCCGACTGTCTATATGGGACAACACCTTTTATGGTTGTCATCGC3070.1272143375116544No Hit
TTTCAGGGCCGCCCCATTCGGTCGGTGAGCTGTTACGCACTCCTTAGCTGATGCCGGCTTCCATGGCCACAGCCC3060.12679995856210505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)