FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241325
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTAGCTGATGCCGGCTTCCATGGCCACAGCCCGACTGTCTATATGGGACAACACCTTTTATGGTTGTCATC5270.21837770641251422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.17279602196208432No Hit
CATATAGACAGTCGGGCTGTGGCCATGGAAGCCGGCATCAGCTAAGGAGTGCGTAACAGCTCACCGACCGAATGG3990.1653372008701958No Hit
GAGTATGACTATCGGCATATTAACGATACCAGGTCCGACTACGTCCATTTTCAGGGCCGCCCCATTCGGTCGGTG3730.15456334818191236No Hit
GCTAAGGAGTGCGTAACAGCTCACCGACCGAATGGGGCGGCCCTGAAAATGGACGTAGTCGGACCTGGTATCGTT3350.13881694809903658No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3030.12555682171345695Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCAGGTCCGACTACGTCCATTTTCAGGGCCGCCCCATTCGGTCGGTGAGCTGTTACGCACTCCTTAGCTGATG2770.11478296902517353No Hit
GTCTATATGGGACAACACCTTTTATGGTTGTCATCGCCCAGCGATTTGTAGCCCTAAGTCAACGTTTGGTTCATC2740.11353983217652544No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2670.11063917952967989Clontech Universal Primer Mix Long (96% over 26bp)
CCTTAGCTGATGCCGGCTTCCATGGCCACAGCCCGACTGTCTATATGGGACAACACCTTTTATGGTTGTCATCGC2660.11022480058013052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.10318035843779136No Hit
ATCCTGAGGGAAACTTCGGGGGAAACCAGCTACTAAACGGCTCGATTGGTCTTTCGCCCCTATACTCAGATTTAA2440.10110846369004456No Hit
GTCCCATATAGACAGTCGGGCTGTGGCCATGGAAGCCGGCATCAGCTAAGGAGTGCGTAACAGCTCACCGACCGA2430.10069408474049518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)