FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251863
Sequences flagged as poor quality0
Sequence length75
%GC29

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60782.413216709083907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40291.599679190671119No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.8619765507438568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16840.6686174626681967No Hit
GCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTAATTTCGAGCA8080.32080932888117747No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.3045306376879494No Hit
GCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAACTCTCGAGCA6190.24576853289288222No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.23425433668303802No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.23187208919134608No Hit
GTACATGGGTACTCCATGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAAT4720.1874034693464304No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.1766833556338168No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.15683129320305086No Hit
GTATCAACGCAGAGTACATGGGTACTCCATGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAG3690.1465082207390526No Hit
TGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAA3200.12705319955690197No Hit
TGAACATACAGCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTA3160.12546503456244068No Hit
CACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAACTCTCGAGCATATCTTCAGGCTGTATGTTCATGGAGTA3010.11950941583321092No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.11395083835259645No Hit
CATACAGCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTAATTT2860.11355379710398113No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.1103774671150586No Hit
TTCCTACTCCATGAACATACAGCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTT2760.10958338461782793No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2720.10799521962336668Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)