FastQCFastQC Report
Wed 8 May 2024
EGAF00002147041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285956
Total Bases21.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC30

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98493.444236176194939No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA77772.7196491767964304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64532.2566408818139854No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA58442.0436710542880725No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48941.7114521115136596No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA36071.2613828700919023No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA33801.182000027976332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32601.1400355299416693No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA28861.0092461777336372No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA27490.9613367091440641No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.9379065310747108No Hit
ATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20820.7280840409013974No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA19180.6707325602540252No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15380.5378449831442599No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.48049350249688766No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13540.47349941949111046No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA13300.465106519884178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.4549650995258012No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.4130006014911385No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11190.39131894417322943No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA11170.39061953587265175No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10030.35075326273972224No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9610.33606568842759027No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.32522485976863574No Hit
GTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA8310.290604148890039No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7760.27137042062415195No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA7190.25143728405768717No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA7140.2496887633062429No Hit
TCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7080.24759053840450979No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6660.23290296409237782No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6350.22206213543342332No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.22171243128313445No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA6020.21052189847389108No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.21017219432360223No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA5740.20073018226580314No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC5680.19863195736407No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5490.19198757850858175No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5480.19163787435829288No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA5440.19023905775713745No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.18534319965309348No Hit
ACGCAGAGTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAAAAAAAA4930.1724041460924058No Hit
TCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4890.17100532949125039No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4490.15701716347969616No Hit
GCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCC4480.15666745932940732No Hit
AACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4460.1559680510288296No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4440.1552686427282519No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.1538698261270965No Hit
ACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA4380.15317041782651877No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA4350.1521213053756522No Hit
CAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4250.14862426387276365No Hit
CAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4230.14792485557218593No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.14687574312131937No Hit
GTATCAACGCAGAGTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAA4150.1451272223698751No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAG4130.1444278140692974No Hit
CACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAACTCTCGAGCATAT4040.1412804767166977No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA3900.1363846186126537No Hit
AACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3890.13603491446236485No Hit
GTATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAA3790.13253787295947628No Hit
GTACATGGGAAGCACTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAA3760.13148876050860972Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAAA3700.12939053560687658No Hit
ACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3670.12834142315601002No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAA3500.12239645260109946No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA3400.11889941109821092No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAA3310.11575207374561122No Hit
GTACATGGGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.11575207374561122No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA3290.11505266544503351No Hit
TATCAACGCAGAGAACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.10840828658954524No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAC3080.10770887828896752No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC3070.10735917413867868No Hit
GCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAATC3050.10665976583810097No Hit
GGAATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.10491124508665668No Hit
TATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA2970.10386213263579012No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2930.1024633160346347No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAA2900.10141420358376814No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)