FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308402
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.725027723555619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.5369614983041614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7890.2558349167644827No Hit
GTTCTAAGTTGGTTGTTAATCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACACCAGGTGCTGGGTGCC7700.24967412662693497No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG7190.2331372688893068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.21400639425165854No Hit
CTACAGAGGTGTAGTGCTCTGCCAGCCATTAAACCCTAGCTCCGGACAAACCGTTTTCAGGGTGAGAGACTGTTA6370.20654859566410075No Hit
CCTCTGTAGTGTCCGGTGCGCTCCCGACGTCCCTTGAAAATCCGCTGGAAGGAATAAGATTCGCGCCAGGTCGTA5900.19130874637648265No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACA4310.13975266048858306No Hit
GTGTACGACCTGGCGCGAATCTTATTCCTTCCAGCGGATTTTCAAGGGACGTCGGGAGCGCACCGGACACTACAG4250.13780714781356798No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGTGTACGACCTGGCGCGA4240.1374828957010655No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT4170.13521313091354792No Hit
GTCGTACACATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC4170.13521313091354792No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGTGTACGACCTGGCGCGAATCTTATTCCTTCCAGCGG4110.13326761823853284No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.12937659288850267No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACACCAGGT3880.12580981965097504No Hit
TGCCAAGGGCGTCCACACCAGGTGCTGGGTGCCGCCGGCCGGTTGCCCGGCTAGTGACTCCCTGCTCCCGGCGCT3870.1254855675384725No Hit
GTGCTGGGTGCCGCCGGCCGGTTGCCCGGCTAGTGACTCCCTGCTCCCGGCGCTTCGACCCAAGGCCCAACGCAC3530.11446099571338707No Hit
GGGTAGAGTTCTCTTTTCTACTTAACAGTCTCTCACCCTGAAAACGGTTTGTCCGGAGCTAGGGTTTAATGGCTG3350.10862445768834184No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT3270.10603044078832174No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.10376067600080414No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGGGTCGAAGCGCCGGGAGCAGGGAGTCACTAGCCGGGCAACCGGCCG3200.10376067600080414No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)