FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207087
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41311.9948137739211054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26711.2897960760453335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.7697248016534114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.5760863791546548No Hit
GCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTAATTTCGAGCA6230.30083974368260685No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.2699348582962716No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4890.23613263990496747Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4730.22840641855838367Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.22212886371428434No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.21343686469937756No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3720.1796346463080734TruSeq Adapter, Index 3 (96% over 28bp)
GCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAACTCTCGAGCA3480.16804531428819772No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.1608019817757754No Hit
GTACATGGGTACTCCATGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAAT3230.15597309343416052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2720.13134576289192465No Hit
GTATCAACGCAGAGTACATGGGTACTCCATGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAG2570.12410243037950233No Hit
TGAACATACAGCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTA2270.10961576535465771No Hit
TGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAA2210.10671843234968878No Hit
CTCCATGAACATACAGCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAG2130.10285532167639687No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.10092376633975093No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)