FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207087
Sequences flagged as poor quality0
Sequence length75
%GC30

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40991.9793613312279381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27091.30814585174347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.7576525807993741No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.5519419374465804No Hit
GCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTAATTTCGAGCA5710.27572952430620945No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.24096152824658237No Hit
GCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAACTCTCGAGCA4760.22985508506086816No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3950.19074108949378765Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.17915175747391193No Hit
GTACATGGGTACTCCATGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAAT3570.17239131379565112No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3510.16949398079068217Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.16273353711242136No Hit
TGAACATACAGCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTA2690.1298970963894402No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.12748265221863275No Hit
GTATCAACGCAGAGTACATGGGTACTCCATGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAG2420.11685909786708001No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.11541043136459556No Hit
CACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAACTCTCGAGCATATCTTCAGGCTGTATGTTCATGGAGTA2290.11058154302298069No Hit
TGAACATACAGCCTGAAGATATGCTCGAAATTAACCCTCACTAAAGGGAGTCGACTAATCGTGACGCGTGACTAA2250.10864998768633473No Hit
TTCCTACTCCATGAACATACAGCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTT2220.10720132118385026No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.10285532167639687No Hit
CATACAGCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTAATTT2120.10237243284223539No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)