FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388957
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT28130.7232161909928347No Hit
GTGCTGGAAGGCGCCGGCCCTTGCGGGCTAGTGCCGACCTGCTCCAGGGACTTCAGCCCAAGGCCCAACGTGCCC17920.46071930830400276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12970.3334558832981538No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACG10960.2817792198109303No Hit
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAA10210.2624968826888319No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCACGTTG10210.2624968826888319No Hit
GCCTTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCTTTCAGGGTCCGAGTTGTAATTT10160.26121139354735873No Hit
GTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTT9420.24218615425355502No Hit
GTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGGCCGAAACCTGCCAAGAGCGTCCGCCACCCGGTGCTGGAAGG9380.24115776294037647No Hit
TTCCAGCACCGGGTGGCGGACGCTCTTGGCAGGTTTCGGCCGTCCGGCGTGCGCTTAACAACCAACTTAGAACTG8960.23035965415200138No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACATGGAGAAGGCGGCCGGTCGCATACGG8640.22213252364657277No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGACCGGTACCAACGCCGAAGCATCCTCTACAAATTACAACTCGGAC8520.21904734970703701No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACATGGAGAAGGCGGCCGGTCGCATACGGGATTCTCACCCTC8090.20799214309036732No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA8030.20644955612059945No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCCTTCCGGCGG7970.2049069691508316No Hit
GTCCATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAACGGCCGGTAGAGCCCCGCACCTTTGCGGGGTCC7840.2015646973830012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.19308046904927795No Hit
GTGATGGACCGTTAAGAAGAAAAGAGAACTCTTCCCAGGGCCCCCGCCGACGTCTCCGCTTTCAGTTACGTTGCC7140.1835678494023761No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT6720.17276974061400102No Hit
CCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGCGGGGGTAGAAGGCCCGCCTACGGGGCGCC6250.16068614268415274No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAA6030.15502999046167057No Hit
GTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGT6010.1545157948050813No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGGCCGAAACCTGCCAAGAGCGTCCGCCACCCGG6000.15425869697678662No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA5930.15245901217872412No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5830.14988803389577768No Hit
ACTTATACCTCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTG5690.14628866429965265No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGGCCGAAACCTGCCAAGAGCGTCCGCC5650.1452602729864741No Hit
CTCCACGGCAACGTAACTGAAAGCGGAGACGTCGGCGGGGGCCCTGGGAAGAGTTCTCTTTTCTTCTTAACGGTC5490.14114670773375976No Hit
GTCATAGAGGGTGAGAATCCCGTATGCGACCGGCCGCCTTCTCCATGTGAAGCTCCTTCGACGAGTCGAGTTGTT5440.13986121859228653No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGGCCG5340.13729024030934012No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCC5250.1349763598546883No Hit
CTATGACGTCCTGTTCCAAGGAACTTAGACCGGTACCAACGCCGAAGCATCCTCTACAAATTACAACTCGGACCC4910.1262350336926704No Hit
GCGCACAAGTGCGCACTTTAAAACGGAGTTTCCCTATCTCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCA4880.12546374020778644No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT4880.12546374020778644No Hit
GAGTACGACCTGGCGTGAAAACTATTCCTTCCGGCGGATTTTCAAGGGTCGTCGGGGGCGCACCGGACCCCGCAA4810.12366405540972394No Hit
TAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCTTTCAGGGTCCGAGTTGTAATTTGTAG4700.12083597929848286No Hit
GTCCGGTGCGCCCCCGACGACCCTTGAAAATCCGCCGGAAGGAATAGTTTTCACGCCAGGTCGTACTCATAACCG4690.12057888147018823No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTG4690.12057888147018823No Hit
CTCTAAGGGTTGGGCACGTTGGGCCTTGGGCTGAAGTCCCTGGAGCAGGTCGGCACTAGCCCGCAAGGGCCGGCG4590.11800790318724177No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATA4560.11723660970235784No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCCTTCCGGCGGATTTTCAAGGGTCGT4450.11440853359111676No Hit
GTGCCGACCTGCTCCAGGGACTTCAGCCCAAGGCCCAACGTGCCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT4430.11389433793452747No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAAC4380.11260884879305424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.1118375553081703No Hit
CCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACATGGAGAAGGCGGCCGGTCGCATA4350.1118375553081703No Hit
ACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAACA4270.10978077268181316No Hit
TGCCAAGAGCGTCCGCCACCCGGTGCTGGAAGGCGCCGGCCCTTGCGGGCTAGTGCCGACCTGCTCCAGGGACTT4250.10926657702522388No Hit
GTACCAACGCCGAAGCATCCTCTACAAATTACAACTCGGACCCTGAAAGGGCCAGATTTCAAATTTGAGCTGTTG4210.10823818571204528No Hit
GGTTAATATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAACTGAAAGCGGAGACGTCGGCGGGGGCCCT4190.10772399005545599No Hit
CTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCG4170.10720979439886671No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCAC4170.10720979439886671No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGCACGCCGGACGGCCGAAACCTGCCAAGAG3940.10129654434808989No Hit
GTATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGC3940.10129654434808989No Hit
CCCTGGAGCAGGTCGGCACTAGCCCGCAAGGGCCGGCGCCTTCCAGCACCGGGTGGCGGACGCTCTTGGCAGGTT3920.1007823486915006No Hit
CCGTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCGAGGTTAAAGTGCCGG3910.10052525086320595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)