FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392915
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40261.0246490971329676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.7034600358856241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.36318287670361277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.29141162846926183No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA10210.25985263988394436RNA PCR Primer, Index 27 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.2560350202970108No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGACATCTCCGGATCACAGGATATTTGCCGCCTCCCCGGAGCTTTTC6520.16593919804537877No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.16517567412799206No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAAGAAAGACGTGATAGCCTGCGAAAAGCTCCGG5930.15092322767010677No Hit
TTGCAACACTATGTATTCATGTTGCAATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGACATCTCCGGATC5260.13387119351513688No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGATAGCCTGCGAAAAGCTCCGG5030.1280175101485054No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.1272539862311187No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTTGCAACACTATGTATTCATGTTGCAATACCTACCTTATGGTA4940.12572693839634527No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.11045646004861102No Hit
TTGCAATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGACATCTCCGGATCACAGGATATTTGCCGCCTCCC4300.10943842815876208No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)