FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15767
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA177911.283059554766284TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4712.9872518551404834TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4592.9111435276209807RNA PCR Primer, Index 20 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA1691.071858945899664RNA PCR Primer, Index 7 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1300.8245068814612798RNA PCR Primer, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA990.6278937020358978TruSeq Adapter, Index 7 (96% over 28bp)
GCTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA890.5644700957696455RNA PCR Primer, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA880.5581277351430203TruSeq Adapter, Index 7 (96% over 28bp)
CAGTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA840.5327582926365193RNA PCR Primer, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG670.4249381619838904TruSeq Adapter, Index 7 (96% over 28bp)
AGCTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA590.37419927697088856RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA540.34248747383776246Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA520.32980275258451197TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC510.32346039195788673TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA440.27906386757151014TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA360.22832498255850828RNA PCR Primer, Index 20 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA360.22832498255850828TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA340.21564026130525782TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA320.20295554005200733RNA PCR Primer, Index 20 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC320.20295554005200733RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT310.19661317942538215TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA310.19661317942538215TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG300.1902708187987569TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA300.1902708187987569RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA290.18392845817213166TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA290.18392845817213166TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA280.17758609754550644RNA PCR Primer, Index 20 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA260.16490137629225599Illumina PCR Primer Index 7 (96% over 26bp)
CAGCTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA240.1522166550390055TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG230.14587429441238028RNA PCR Primer, Index 20 (96% over 29bp)
CAGATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA230.14587429441238028RNA PCR Primer, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGTTGGTCAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA210.13318957315912983Illumina PCR Primer Index 7 (96% over 26bp)
CAGTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA200.1268472125325046RNA PCR Primer, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAA190.12050485190587938TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA180.11416249127925414Illumina PCR Primer Index 7 (96% over 26bp)
NTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA180.11416249127925414RNA PCR Primer, Index 7 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACAA180.11416249127925414RNA PCR Primer, Index 7 (96% over 28bp)
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAA180.11416249127925414TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC180.11416249127925414TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA170.10782013065262891TruSeq Adapter, Index 7 (96% over 28bp)
GATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA160.10147777002600367RNA PCR Primer, Index 20 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA160.10147777002600367RNA PCR Primer, Index 7 (96% over 28bp)
GACAGATACACATCTCCGAGCCCACGAGACTGTTGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA160.10147777002600367RNA PCR Primer, Index 20 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)