FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309260
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG16100.520597555454957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCACACCCGGCCATCTGGGCGAGCGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCTGCAAAACCCGGG12220.3951367781155016No Hit
ACCGAGTACCGTCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGA11920.38543620254801786No Hit
GGGTAACCCCGGCAGATAGCGCGATCACGCGCATCCCCCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGAT10880.35180754058074115No Hit
GCGCTGAAGCGCGCGACCCACACCCGGCCATCTGGGCGAGCGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCC10680.3453404902024187No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGACTGGGCTCGGGG10450.33790338226734784No Hit
GTGTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACCGTCCACGCCCGGTCGTACTCATAAC9450.3055681303757356No Hit
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGG9250.2991010799974132No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATTCCCGGTCCGTCCCCCGGCCGGCACG9210.2978076699217487No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9160.2961909073271681Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCGGTGCTCTTCCGAC8500.274849641078704No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT7090.22925693591153073No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6790.2195563603440471Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTC6780.21923300782513097No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6700.21664618767380195Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTGGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT6670.2156761301170536No Hit
GTCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAACAATGTAGG6480.20953243225764728No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATTCCCGGTCCG6470.20920907973873115No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAA6400.20694561210631832No Hit
CTCCTACTCATCGGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCGCGCGCTTCAGCGCCATCCATTTTCG6400.20694561210631832No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCCACACCCGGCCA6210.20080191424691202No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCATTGGCC6120.19789174157666686No Hit
TGGGTAAGCCGATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATCACGCGCATCCCCCGAAAGGGAATCG5790.18722110845243484No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTA5700.18431093578218974No Hit
GTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATTCCCGGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCT5630.1820474681497769No Hit
GTGCTCTTCCGACCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGTTGGCAGGCCGTTAAGCAGAAAAGAT5500.17784388540386728No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG5430.17558041777145444No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5280.1707301299877126Clontech Universal Primer Mix Long (96% over 26bp)
GGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCTCCTCTTCGGCC5280.1707301299877126No Hit
GAGTAGGAGGGCGCGGCGGCCGCTGCAAAACCCGGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTG5230.16911336739313199No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCCA5180.16749660479855139No Hit
AGGTAGGATGGTGCGGCTGCTCCGGTGAGCCGTGCCACGGAATCGGGTGCTCCAAGTGGGCCATTTTTGGTAAGC4980.16102955442022895No Hit
ATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATCACGCGCATCCCCCGAAAGGGAATCGGGTTAAGATTT4900.15844273426889996No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC4730.15294574144732587No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATTCCCGGTCCGTCCCCC4700.1519756838905775No Hit
TACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATTCCCGGTCCGTCCCCCGGCCG4610.1490655112203324No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4600.14874215870141627Clontech Universal Primer Mix Long (96% over 26bp)
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTC4480.1448619284744228No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCCACACCCGGC4390.1419517558041777No Hit
GTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACCGTCCACGCCCGGTCGTACTCATAACCG4350.1406583457285132No Hit
GGCGTGGACGGTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCGGTG4320.13968828817176487No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAA4250.137424820539352No Hit
GAGTACGACCGGGCGTGGACGGTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACCGCGC4030.13031106512319732No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3830.12384401474487487Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3810.12319730970704262Clontech Universal Primer Mix Long (96% over 26bp)
GAAGAGCACCGCACGTCGCGCGGTGTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACCGTC3760.12158054711246201No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGG3600.11640690680980405No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3600.11640690680980405Clontech Universal Primer Mix Long (96% over 26bp)
GGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGACGGTACTCGGTCCTCCG3530.11414343917739118No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGGCAA3470.11220332406389447No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAA3420.11058656146931385No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3200.10347280605315916Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCGATGCCTCTAATCATTGGCTTTACCTG3170.10250274849641079No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCATTACGAGTTCTATCAGGTAAAGCCA3110.10056263338291405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)