FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523223
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339526.489011377557944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219984.204325880169641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138652.6499217350919206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127142.429939050844477No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93751.7917790311205737No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71071.3583118479118845No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43180.8252695313470547No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36770.7027596263925706No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33640.6429380971402251No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21680.4143548735434031No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.39409582529819975No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.3379056348822586No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.30293010819478505No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA12510.23909499391272937RNA PCR Primer, Index 36 (96% over 29bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12290.23489028578636642No Hit
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.20564845199847864No Hit
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC9030.17258415627753365No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.16436586312146062No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.1551919544821233Clontech SMART CDS Primer II A (100% over 21bp)
GGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.14716478442270314No Hit
GATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.1299637057239456No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA6650.1270968592741527No Hit
CAGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.12155428947121974No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.11715845824820392No Hit
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG5600.10702893412560227No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC5570.1064555648356437No Hit
NTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.10511770315907366No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)