FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214118
Sequences flagged as poor quality0
Sequence length75
%GC25

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50932.3785949803379443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31981.4935689666445606No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA25481.1899980384647717RNA PCR Primer, Index 36 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23061.0769762467424504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.6701912029815336No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA8180.3820323373093341RNA PCR Primer, Index 36 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCGCCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA6980.3259884736453731Illumina PCR Primer Index 1 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.2979665418133926No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.2568677084598212No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.25313145088222383No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.20175790919025957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3410.15925797924508917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3040.14197778794870117No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA2470.11535695270831971RNA PCR Primer, Index 36 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACTCGCCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2420.11302179172232134RNA PCR Primer, Index 36 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTCGCCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2200.10274708338392849RNA PCR Primer, Index 36 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)