FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429003
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG23420.5459169283198486Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17940.4181788938538891Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAAAACCACTTTCTGGACATCGCAATGCTATGTTTT11520.2685295907021629No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11380.2652662102596019Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCAAGGTCGGTCCGACCGCTTCCAGTCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT10220.23822677230695358No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT9980.23263240583399183No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCATCG9130.21281902457558574No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAAAACCACTTTCTGGACATCGCAATGCTATGTTTTAATTAG8990.20955564413302472No Hit
GTTCTAAGTTGGTTGTTAAGCGTACACCAGACGTCCGAAGACAGCCAAGACCATCCACAAGCCAGTCCAACCGAG8770.20442747486614316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.19603592515670057No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC7860.18321550198949657No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7410.17272606485269335Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7140.1664324025706114Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAAAACCACTTTCTGGACATCGCAATGCTATGTTTTA7140.1664324025706114No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC6890.16060493749460958No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCATTTTGAGCTGCATTCC6590.15361197940340746No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6590.15361197940340746Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGAAATCACATTGCGTCAAAACCACTTTCTGGACATCGCAATGCTATGTTTTAATTAGACAGTCA6540.15244648638820707No Hit
AAATCACATTGCGTCAAAACCACTTTCTGGACATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT6480.15104789476996663No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5670.13216690792372082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5670.13216690792372082Clontech Universal Primer Mix Long (96% over 26bp)
ACCTTGGATACTGAGGGCGACTTCGGCGTACTCGGTTGGACTGGCTTGTGGATGGTCTTGGCTGTCTTCGGACGT5600.1305352177024403No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAAAACCACTTTCTGGACATCGCAATGCTATGTTT5580.13006902049636015No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5340.12447465402339844Clontech Universal Primer Mix Long (96% over 26bp)
ACCTTGGATACTGAGGGCAACTTCGGCGTACTCGGTTGGACTGGCTTGTGGATGGTCTTGGCTGTCTTCGGACGT5020.11701549872611614No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5010.11678240012307606Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACACCAGACGTCCGAAGACAGCCAAGACCATCCACA4840.11281972387139484No Hit
GTCTTGGCTGTCTTCGGACGTCTGGTGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC4830.11258662526835476No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACACCAGACGTCCGAAGACAGCCAAGACCATCCACAAGCCAG4700.10955634342883383No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT4630.10792465320755332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.1048943713680324No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4460.10396197695587212Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCCTGACAGTCCTTGAAAATCCAAGGGAATGAATAATTTTTGCGCCTGGTCGTACTCATAACCGCAGCAGGTC4360.10163099092547138No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)