FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508931
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52101.0237144131522742No Hit
AGCCATAACAGTGCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTAT29480.5792533761944153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29210.5739481383527433No Hit
GTGTAGACCCGAAACCAAGTGATCTACCCATGGCCAGGATGAAGGTGCGGTAACACGCCCTGGAGGTCCGAACCC21900.43031373604673323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21490.422257634139009No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG21410.42068571181555064Clontech Universal Primer Mix Long (96% over 26bp)
GTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA19850.3900332265081121No Hit
GTGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGG19430.38178063430995557No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCA18880.3709736683361792No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17430.3424825762234959Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATT17320.34032118302874065No Hit
CTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCC17230.33855277041485No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCG15920.31281254236821887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15910.3126160520777866No Hit
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGCTAGTTTTTGACTCATAGA15710.3086862462691406No Hit
GGTCTACACCCAGCGACTGTCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATCCGGTTAACCTCGCCAC15570.3059353822030884No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAAC15540.30534591133179156No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGCTAGTTTTT14230.2796056832851605No Hit
GTGCGAGCTTGGGAGACAGACATCGGGTGCTAACGTCCGGTGTCAAGAGGGAAACAACCCAGACCGCCAGCTAAG14030.2756758774765145No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCT13800.27115660079657167No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG13690.26899520760181633No Hit
CCCTAGTAGTTTGACCACTTTACTGTGGAAGGATGAACGGGTTGCAATAAAATGGTGGCTGCGACTGTTTAATAA13000.25543737756198776No Hit
CCTTAGAATACTCATCTCGCCCACCTGTGTCGGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTC12400.2436479601360499No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGTGTGCATACA12160.2389321931656747No Hit
GGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCGTCGTA12140.23853921258481012No Hit
CCGTAAGCCCAAGGTTTCCTGTTCAACGTTCATCGGAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCG11510.22616032428757535No Hit
GTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAACCCGTTCATCCTTCCACAGTAAAGTGGTCA11420.22439191167368464No Hit
GACTCACCCTGCTCCGATGAACGTTGAACAGGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATC10760.21142355250515296No Hit
GGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATTCCCAAAG10520.20670778553477778No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCC10490.2061183146634809No Hit
TCCTGAAACCGTGTGCATACAAACAGTCGGAGCCTCTTCGTGGGGTGACGGCGTACCTTTTGTATAATGGGTCAG10370.20376043117829334No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10350.20336745059742872Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCTTACGGCGTGCGGGCTTTTCACCCGCATTATCGCTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA9880.1941324069471107No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT9780.19216750404278773No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAACCC9670.19000611084803246No Hit
TGGTAGGAGAGCGTTCTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTG9490.18646928562025109No Hit
GTTCGGACTCGATTTCTCTACGGCTCCCCTATCCGGTTAACCTCGCCACTGAATGTAAGTCGCTGACCCATTATA9320.183128950682902No Hit
ACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGGGAAACCC9170.18018159632641753No Hit
CTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAGCGATA9110.17900265458382372No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGC9100.17880616429339144No Hit
ATTGCATACTGAATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAA9070.17821669342209456No Hit
ACCCACAGCTCATCCCCTAATTTTTCAACATTAGTGGGTTCGGACCTCCAGGGCGTGTTACCGCACCTTCATCCT8990.17664477109863616No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA8950.17585880993690697No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT8910.1750728487751778No Hit
GTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT8890.1746798681943132No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAACCCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA8810.1731079458708548No Hit
GGCTAAAACAGTCAGGATGTTGGCTTAGAAGCAGCCACCATTTAAAGAAAGCGTAATAGCTCACTGATCGAGTCG8730.17153602354739642No Hit
GTTTTAGCCTTCCCACTTCGTTTTCCACTTAGCCAATCTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTC8630.16957112064307342No Hit
GGTCTACACCCAGCGACTATCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATCCGGTTAACCTCGCCAC8620.16937463035264114No Hit
GAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCG8620.16937463035264114No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.16917814006220883No Hit
AACTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCG8540.16780270802918273No Hit
ATACGAGACCGTACCGCAAACCGACACAGGTGGGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCGGGAGAA8510.16721323715788583No Hit
CCTATGTATTCAGTATGCAATGACCTAAAAGGCCGGGTTTCCCCATTCGGAAATCTGCGGATCAAAGCTCGTTTG8340.16387290222053677No Hit
GTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCG7930.15581680031281253No Hit
GTGGTACTAAGCGTACGACAAGTAGGGCGGGACACGAGAAATCCTGTCTGAACATGGGGGGACCATCCTCCAAGG7860.15444136827978647No Hit
AGCTCGCACTCATCGGTATTCGGAGTTTGCAATGGTTTGGTAAGTCGCAATGACCCCCTAGCCATAACAGTGCTC7820.15365540711805725No Hit
ATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGTGTG7810.15345891682762494No Hit
GCTGTGGGTAGGGGTGAAAGGCTAAACAAACTTGGAAATAGCTGGTTCTCTCCGAAAACTATTTAGGTAGTGCCT7650.1503150721807082No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.14599228579119763No Hit
GGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAAT7350.14442036346773926No Hit
CCCCTATCCGGTTAACCTCGCCACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCCGTCACCCCACGA7330.14402738288687464No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6930.1361677712695827Clontech Universal Primer Mix Long (96% over 26bp)
GAATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGA6850.1345958489461243No Hit
ATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCGATGAACGT6580.12929061110445228No Hit
CTAGTAGTTTGACCACTTTACTGTGGAAGGATGAACGGGTTGCAATAAAATGGTGGCTGCGACTGTTTAATAAAA6580.12929061110445228No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGC6540.1285046499427231No Hit
CTATTTAGGTAGTGCCTCAAGTATCACCATCGGGGGTAGAGCACTGTTATGGCTAGGGGGTCATTGCGACTTACC6530.12830815965229078No Hit
CTCCGAATACCGATGAGTGCGAGCTTGGGAGACAGACATCGGGTGCTAACGTCCGGTGTCAAGAGGGAAACAACC6210.12202047035845724No Hit
CATACAAACAGTCGGAGCCTCTTCGTGGGGTGACGGCGTACCTTTTGTATAATGGGTCAGCGACTTACATTCAGT6170.12123450919672804No Hit
GTTTGTATGCACACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTG6130.12044854803499884No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6010.11809066454981126Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5990.11769768396894667Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5930.11651874222635289Clontech Universal Primer Mix Long (96% over 26bp)
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTA5860.1151433101933268No Hit
GGGTAGGGGTGAAAGGCTAAACAAACTTGGAAATAGCTGGTTCTCTCCGAAAACTATTTAGGTAGTGCCTCAAGT5840.1147503296124622No Hit
GTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCTCGTGTC5760.11317840728900383No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCG5760.11317840728900383No Hit
GGCTTAGCCCCGTTACATCTTCCGCGCAGGACGACTCGATCAGTGAGCTATTACGCTTTCTTTAAATGGTGGCTG5750.11298191699857152No Hit
CCATAACAGTGCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATTT5750.11298191699857152No Hit
CCCCTACCCACAGCTCATCCCCTAATTTTTCAACATTAGTGGGTTCGGACCTCCAGGGCGTGTTACCGCACCTTC5670.11140999467511314No Hit
CCCTGGAGGTCCGAACCCACTAATGTTGAAAAATTAGGGGATGAGCTGTGGGTAGGGGTGAAAGGCTAAACAAAC5650.11101701409424852No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGG5600.11003456264208704No Hit
GCGCAGGACGACTCGATCAGTGAGCTATTACGCTTTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGACTG5580.10964158206122246No Hit
GGTTAATATTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGC5540.10885562089949326No Hit
ACCTAAATAGTTTTCGGAGAGAACCAGCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTACCCACAGCTCATCCC5490.10787316944733177No Hit
GGATGAAGGTGCGGTAACACGCCCTGGAGGTCCGAACCCACTAATGTTGAAAAATTAGGGGATGAGCTGTGGGTA5440.10689071799517026No Hit
CAGTAAAGTGGTCAAACTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCG5410.10630124712387337No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTAACAATAACGGT5380.10571177625257648No Hit
ACCCTGCTCCGATGAACGTTGAACAGGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATCGCTAC5270.1035503830578212No Hit
GTATGTGATAGTCGGACTGATAGTGGAAGCCTTTGGAAACTGGCGCCATAGTGGGTGATAGCCCCGTACACGAAA5250.10315740247695661No Hit
GAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGGGAAACCCGGCCTTTTAGGTCATTGCATACTGAAT5170.1015854801534982No Hit
GTATGCAATGACCTAAAAGGCCGGGTTTCCCCATTCGGAAATCTGCGGATCAAAGCTCGTTTGCTAGCTCCCCGC5150.10119249957263363No Hit
TCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCGATGAACGTTGAACAGGAAACCTTGGGCTTACGGCGTG5110.10040653841090443No Hit
ACCCATGGCCAGGATGAAGGTGCGGTAACACGCCCTGGAGGTCCGAACCCACTAATGTTGAAAAATTAGGGGATG5110.10040653841090443No Hit
CTGTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGA5090.10001355783003983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)