FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508931
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATAACAGTGCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTAT21930.43090320691803013No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG15200.2986652414570934Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGACCCGAAACCAAGTGATCTACCCATGGCCAGGATGAAGGTGCGGTAACACGCCCTGGAGGTCCGAACCC15170.2980757705857965No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA13610.26742328527835796No Hit
GTGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGG13510.26545838237403496No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCA13210.25956367366106603No Hit
ATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATT12880.2530794940768002No Hit
CTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCC12700.24954266884901882No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12660.24875670768728964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGCTAGTTTTTGACTCATAGA11660.2291076786440598No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAAC11550.2269462854493045No Hit
CCCTAGTAGTTTGACCACTTTACTGTGGAAGGATGAACGGGTTGCAATAAAATGGTGGCTGCGACTGTTTAATAA11490.22576734370671073No Hit
GGTCTACACCCAGCGACTGTCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATCCGGTTAACCTCGCCAC11350.22301647964065854No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCT11090.21790773208941883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.20965513989126228No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG10610.2084761981486685No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCG10580.2078867272773716No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTAGCTGTTGGAATAGCTAGTTTTT10310.20258148943569954No Hit
GTGCGAGCTTGGGAGACAGACATCGGGTGCTAACGTCCGGTGTCAAGAGGGAAACAACCCAGACCGCCAGCTAAG9610.18882716910543867No Hit
GTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAACCCGTTCATCCTTCCACAGTAAAGTGGTCA8990.17664477109863616No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGTGTGCATACA8630.16957112064307342No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCC8440.16583780512485974No Hit
CCGTAAGCCCAAGGTTTCCTGTTCAACGTTCATCGGAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCG8370.16446237309183367No Hit
TCCTGAAACCGTGTGCATACAAACAGTCGGAGCCTCTTCGTGGGGTGACGGCGTACCTTTTGTATAATGGGTCAG8280.162693960477943No Hit
GGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCGTCGTA8270.16249747018751068No Hit
CCTTAGAATACTCATCTCGCCCACCTGTGTCGGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTC7850.15424487798935416No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCATTTTATTGCAACCC7630.1499220915998436No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT7560.1485466595668175No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7510.147564208114656Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA7480.1469747372433591No Hit
GACTCACCCTGCTCCGATGAACGTTGAACAGGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATC7330.14402738288687464No Hit
GTTCGGACTCGATTTCTCTACGGCTCCCCTATCCGGTTAACCTCGCCACTGAATGTAAGTCGCTGACCCATTATA7300.14343791201557773No Hit
CCTATGTATTCAGTATGCAATGACCTAAAAGGCCGGGTTTCCCCATTCGGAAATCTGCGGATCAAAGCTCGTTTG7300.14343791201557773No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT7290.14324142172514545No Hit
GGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATTCCCAAAG7090.1393116159164995No Hit
ACCCACAGCTCATCCCCTAATTTTTCAACATTAGTGGGTTCGGACCTCCAGGGCGTGTTACCGCACCTTCATCCT6920.13597128097915043No Hit
ATTGCATACTGAATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAA6710.13184498488007215No Hit
ACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGGGAAACCC6450.1267362373288324No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGC6410.1259502761671032No Hit
AACTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCG6400.1257537858766709No Hit
GGCTAAAACAGTCAGGATGTTGGCTTAGAAGCAGCCACCATTTAAAGAAAGCGTAATAGCTCACTGATCGAGTCG6320.12418186355321252No Hit
GTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCG6320.12418186355321252No Hit
GGCTTACGGCGTGCGGGCTTTTCACCCGCATTATCGCTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA6280.12339590239148332No Hit
ATACGAGACCGTACCGCAAACCGACACAGGTGGGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCGGGAGAA6220.12221696064888952No Hit
GTTTTAGCCTTCCCACTTCGTTTTCCACTTAGCCAATCTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTC6170.12123450919672804No Hit
GGTCTACACCCAGCGACTATCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATCCGGTTAACCTCGCCAC6160.12103801890629574No Hit
GTGGTACTAAGCGTACGACAAGTAGGGCGGGACACGAGAAATCCTGTCTGAACATGGGGGGACCATCCTCCAAGG6010.11809066454981126No Hit
TGGTAGGAGAGCGTTCTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTG5960.11710821309764978No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAACCCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA5960.11710821309764978No Hit
GCTGTGGGTAGGGGTGAAAGGCTAAACAAACTTGGAAATAGCTGGTTCTCTCCGAAAACTATTTAGGTAGTGCCT5940.11671523251678519No Hit
GTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT5920.11632225193592058No Hit
GTTTGTATGCACACGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTG5780.11357138786986841No Hit
CCCCTATCCGGTTAACCTCGCCACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCCGTCACCCCACGA5720.11239244612727463No Hit
ATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGTGTG5700.11199946554641003No Hit
CTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAGCGATA5680.11160648496554543No Hit
GAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCG5640.11082052380381624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.10748018886646715No Hit
AGCTCGCACTCATCGGTATTCGGAGTTTGCAATGGTTTGGTAAGTCGCAATGACCCCCTAGCCATAACAGTGCTC5390.10590826654300878No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC5340.1049258150908473Clontech Universal Primer Mix Long (96% over 26bp)
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTA5100.10021004812047211No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)