FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302011
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG43191.4300803613113429Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG38791.2843903036644362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20190.6685186963388751Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15620.5171997046465194Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13660.45230140624017007Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA12950.4287923287562373Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12050.3989920896920973Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11860.39270092811188995Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10650.3526361622589906Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10350.34270274923761057Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.2983335044087798No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGG8930.29568459426974514No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8760.29005566022429646Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG8660.28674452255050314No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8190.27118217548367446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8060.2668776965077431Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT7610.2519775769756731No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT6670.2208528828420157No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5800.19204598508001366Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG4940.16357020108539094No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC4920.16290797355063225No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCA4890.16191463224849426No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAG4780.1582723808073216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.15761015327256292No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC4680.15496124313352824Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4560.15098787792497625Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.14800785401856226No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4410.14602117141428622Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4320.14304114750787222Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT4220.13973000983407888Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA4070.1347633033233889No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3810.1261543453715262Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTAC3680.12184986639559485No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT3680.12184986639559485No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT3570.11820761495442218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCT3550.11754538741966351No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG3550.11754538741966351Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG3510.11622093235014619No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA3500.11588981858276685No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3450.11423424974587018No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3440.11390313597849085Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG3320.10992977076993884Clontech Universal Primer Mix Long (96% over 26bp)
GCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA3320.10992977076993884No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3150.10430083672449016Clontech Universal Primer Mix Long (96% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT3150.10430083672449016Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA3100.1026452678875935Clontech Universal Primer Mix Long (96% over 26bp)
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGG3090.10231415412021416No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3080.10198304035283483Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)