FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378320
Sequences flagged as poor quality0
Sequence length20-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180194.762899133009093No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33240.8786212729964051No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC19860.5249524212307042No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18050.47710932543878193No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA16790.44380418693169804No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16400.4334954535842673No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC15400.4070628039754705No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG13010.3438887714104462No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA12080.3193064072742652No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT11970.3163988158172975No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG11470.3031824910128991No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11110.2936667371537323No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT10840.28652992175935715No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9390.24820257982660182No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8780.23207866356523577Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG8610.22758511313174032No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT8450.22335588919433283No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT7540.19930217805032777No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC7300.19295834214421653No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC7240.19137238316768873No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA7230.19110805667160075No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6750.1784203848593783No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC6740.17815605836329035No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG6710.17736307887502642No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC6480.17128356946500317No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT6190.1636181010784521No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA6120.16176781560583633No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6100.1612391626136604No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6000.1585958976527807No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT5970.15780291816451683No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA5870.15515965320363712No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG5580.14749418481708607No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA5550.14670120532882217No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC5540.1464368788327342No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5350.1414146754070628No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5280.13956438993444703No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG5210.13771410446183127No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT5120.13533516599703954No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA5010.1324275745400719No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT4930.13031296257136815No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC4790.1266123916261366No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4780.12634806513004865No Hit
ATGGGAAGCAGTGGTATCAACGC4560.12053288221611336No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC4530.11973990272784944No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA4520.11947557623176147No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT4440.11736096426305774No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC4120.10890251638824276No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT4000.10573059843518715No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3970.10493761894692324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3870.10229435398604356No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG3860.10203002748995559No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC3790.10017974201733983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)