FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399363
Sequences flagged as poor quality0
Sequence length20-100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49221.2324626968447252No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG16850.4219219106426985No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10090.2526523488655684No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9040.22636047906290768No Hit
GTAATAAACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA6710.16801756797700337No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6600.16526318161672462No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6560.16426158657662326No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5950.14898726221507752No Hit
GTTTATTACTGTGCGAGAGATGGGAGCTTCTATGAGGGGGACTTATGGGG5920.1482360659350015No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5510.13796971677396255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5470.13696812173386116No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5240.13120895025327833No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5140.12870496265302495No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5090.12745296885289825No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4980.1246985824926195No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4820.12069220233221405No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC4540.11368103705150451No Hit
ATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTTTCCA4520.11318023953145384No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4470.11192824573132713No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG4240.1061690742507443No Hit
GAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA4150.10391548541051625No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG4020.10066030153018683No Hit
GATCTATGCTGCATCCAGTTTGCAAAGCGGGGTCCCGTCAAGGTTCAGTG4020.10066030153018683No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT4010.10040990277016149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)