FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318414
Sequences flagged as poor quality0
Sequence length20-100
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170045.340217452750194No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53911.6930788219110968Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGC29570.9286651968820466No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT27240.8554900224236371Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22100.6940649594553003No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20340.6387910079330683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC18390.5775499820987771Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18280.5740953601286376No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA17310.543631875482862Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16270.5109699950379066Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13470.4230341630707192Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12950.4067032228482416No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12200.383148982142745No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9210.2892460758634985Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8970.28170871883773957No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA8430.264749665529782Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC8060.25312957344840364Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8040.2525014603629237Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7980.25061712110648404Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG7640.23993919865332555Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC7490.23522835051222624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7360.23114561545660683Clontech Universal Primer Mix Long (95% over 21bp)
ATGGGAAGCAGTGGTATCAACGCAGAGT7340.23051750237112692Clontech Universal Primer Mix Long (100% over 23bp)
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7120.22360825843084786No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6680.20978977055028988Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.20036807426809122No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6180.19408694341329213Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT5990.188119869101233Clontech Universal Primer Mix Long (96% over 26bp)
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5950.18686364293027316No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATG5640.17712789010533456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.17178892887875533Clontech SMART CDS Primer II A (100% over 26bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT5390.1692764765368357Clontech SMART CDS Primer II A (96% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT5130.16111100642559686Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT5080.1595407237118971Clontech SMART CDS Primer II A (100% over 26bp)
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5010.1573423279127174No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4910.15420176248531786Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAG4890.15357364939983795No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC4840.15200336668613817Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4770.1498049708869585Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA4520.1419535573184596Clontech Universal Primer Mix Long (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.14101138769023974Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA4430.13912704843380003Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4350.13661459609188037Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.13473025683544065No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4240.13315997412174088Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4210.132217804493521Clontech Universal Primer Mix Long (95% over 21bp)
ATGGGAAGCAGTGGTATCAAC4140.13001940869434134No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG4130.1297053521516014Clontech Universal Primer Mix Long (96% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGA4100.1287631825233815Clontech Universal Primer Mix Long (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.11243224230090385No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT3560.11180412921542393Clontech SMART CDS Primer II A (100% over 26bp)
ATGGGAAGCAGTAGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.111490072672684No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3450.10834950724528444Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3420.10740733761706457No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA3390.10646516798884471Clontech Universal Primer Mix Long (96% over 26bp)
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3340.10489488527514494No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC3330.10458082873240498Clontech Universal Primer Mix Long (95% over 21bp)
ATACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.10238243293322528No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG3190.10018403713404561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)