FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145908
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.7031828275351591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.3461085067302684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.2755160786248869No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3120.21383337445513612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2710.1857334758889163Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.13090440551580448No Hit
GTTCTAAGTTGGTCGTTAATCGCCCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCCCACCCGGTGCTGGATGGC1510.10348987032924857No Hit
ATCCAGCACCGGGTGGGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGGGCGATTAACGACCAACTTAGAACTGG1460.10006305343092908No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)