FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299729
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14370.47943308788939343No Hit
ATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTC8130.2712450246722873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.26657413863857016No Hit
GTACTGATCCGCTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCT6920.2308752239523036No Hit
GGTCTAGGACACGCGACTCAATCGCCCTATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGC6580.21953164358470487No Hit
GATCAGTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAACCATGTGCCTACA5880.1961772134161192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.18316545946505008No Hit
TCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCCAC5430.18116365116488561No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGAATGGGCACGGGG4920.16414828061348752No Hit
GTACATGGGTAGATCACTCCGCTTCGGGTCTAGGACACGCGACTCAATCGCCCTATTCAGACTCGCTTTCGCTAC4790.15981102929646449No Hit
TTCCAAGGGCGCATGGTGGATGCCTTGGCAACAGGAGCCGAAGAAGGACGTGGGAATCTGCGATAAGCCTGGTGG4140.13812477271134926No Hit
GTGGATGCCTTGGCAACAGGAGCCGAAGAAGGACGTGGGAATCTGCGATAAGCCTGGTGGAGTCGATAACCGGAC4040.13478842554440845No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.1321193478108558No Hit
GGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCCACAAGGAGGCTC3600.1201084980098689No Hit
GGGCTAAACCGTATGTGTGTGATACCCGGCAGGGGTTGCATGTGCGGTGTTGTGGGCCCCTCCTTGTCATGTCTG3440.11477034254276364No Hit
CACATAGACCGGGTGGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAGGAAGAGAAAACAAAAGTGATT3410.11376943839268139No Hit
GTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCGTAGTCGAAATGTGTTCCGCCGCTGTTGGAGGGTTGCCCGA3320.11076672594243467No Hit
CATATAGACGAACCAGTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCGTAGTCGAAATGTGTTCCGCCGCTGT3310.11043309122574059No Hit
GTTGTGGGCCCCTCCTTGTCATGTCTGCCGGCATGGCGCAGTGAGGTGCGGGCATATAGACGAACCAGTGTGGAT3280.10943218707565834No Hit
ATGCTGGACCGTAGAGGGTGAGAGTCCCGTAGTCGAAATGTGTTCCGCCGCTGTTGGAGGGTTGCCCGAGTAGCA3270.10909855235896426No Hit
GTTGGAGGGTTGCCCGAGTAGCACGGGGCCCGAGGAATCCCGTGTGAATCTGCCAGGACCACCTGGTAAGCCTGA3260.10876491764227017No Hit
GTATCAACGCAGAGTACATGGGTAGATCACTCCGCTTCGGGTCTAGGACACGCGACTCAATCGCCCTATTCAGAC3230.10776401349218794No Hit
GCGCAGTGAGGTGCGGGCATATAGACGAACCAGTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCGTAGTCGAA3200.1067631093421057No Hit
GCCCTATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCT3140.10476130104194123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)