FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288356
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAACCTTGAAATCGGTTTGTCCGGAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTCTGTCGGGTCC17210.5968316941558351No Hit
GTTGTAGACTGTTAAGGAGAAAAGATAACTCTTCCCGGGGCCCCCGCCGATGTCTCCAAGTTCATTTACGTTGCC15120.5243518428609081No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTCG12420.43071758520717446No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATCCACCAACAGCGCCTGG11860.4112971465826964No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAA11030.38251328219284497No Hit
ATCCTAAGAGATAGGGAAGCTCCGTTTCAAAGTGCACGATTCTTCGTGCCGCCTATCGAAAGGGAATCCGGTTAA10890.37765817253672546No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAAAAATTATTCATTCCCTTGG10460.36274605002150123No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT9800.3398576759283663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9090.31523533410090304No Hit
CTCCTTAACAGTCTACAACCTTGAAATCGGTTTGTCCGGAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTC7690.2666842375397078No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT7670.2659906504459765No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC7510.2604419536961256No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATCCACCAA7490.25974836660239425No Hit
GTCCCAGCTGAGCACCGAGGCTTTCTACCGAGGCCCGACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT7470.2590547795086629No Hit
GGCTGGTAGAGCTCGACACTTCTGTCGGGTCCGGTGCGTCCTCGACGATCCTTGAAAATCCAAGGGAATGAATAA7460.2587079859617972No Hit
CAGCTGGGACTGCTTGGGGCAACTCTTGTGGATCTAGCCAGGCGCTGTTGGTGGATGACCTTGGCTGCCCTCTCG7180.2489977666495582No Hit
CTACCAGCCATTGAACCTTAGCTCCGGACAAACCGATTTCAAGGTTGTAGACTGTTAAGGAGAAAAGATAACTCT6840.23720678605612505No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATC6570.22784336029075172No Hit
GTTAAGGAGAAAAGATAACTCTTCCCGGGGCCCCCGCCGATGTCTCCAAGTTCATTTACGTTGCCGTTAAAAATC6560.22749656674388602No Hit
GTCCCAGCTGAGCACCGCGGCTTTCTACCGAGGCCCGACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT6440.22333504418149785No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC6060.21015688940060206No Hit
GTCCTCGACGATCCTTGAAAATCCAAGGGAATGAATAATTTTTGCACTTGGTCGTACTCATAACCGCAGCAGGTC5980.2073825410256766No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAAAAATTATTCA5850.20287422491642276No Hit
GAGTACGACCAAGTGCAAAAATTATTCATTCCCTTGGATTTTCAAGGATCGTCGAGGACGCACCGGACCCGACAG5840.2025274313695571No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG5800.20114025718209438No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT5790.20079346363522868No Hit
GTCGAGGACGCACCGGACCCGACAGAAGTGTCGAGCTCTACCAGCCATTGAACCTTAGCTCCGGACAAACCGATT5700.19767232171343757No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC5670.19663194107284052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.1903896572292583No Hit
AGATAGGGAAGCTCCGTTTCAAAGTGCACGATTCTTCGTGCCGCCTATCGAAAGGGAATCCGGTTAATATTCCGG5150.17859867663582515No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCTATATTTAGCTTTAGATGGAATTTACCACCCATTTTGAGCTGCATTCC5120.17755829599522813No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAAAAATTATTCATTCCCTTGGATTTTCAAGGATCGT5110.17721150244836245No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCC5020.17409036052657131No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA5010.17374356697970564No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCA4920.17062242505791453No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA4760.16507372830806363No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT4730.1640333476674666No Hit
TGATTGGACCGCTCGGCGATTGAGAGTTTCTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAAC4670.16195258638627252No Hit
GTCCAGCGTGGGTAACACTTCTCTAAATTACAACTCGGACGGCCTCGGGGACCGCCAGATTTTAAATTTGAGCTT4580.15883144446448139No Hit
CCCCCGAGAGGGCAGCCAAGGTCATCCACCAACAGCGCCTGGCTAGATCCACAAGAGTTGCCCCAAGCAGTCCCA4540.15744427027701868No Hit
GTGCTCAGCTGGGACTGCTTGGGGCAACTCTTGTGGATCTAGCCAGGCGCTGTTGGTGGATGACCTTGGCTGCCC4470.15501671544895892No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG4430.1536295412614962No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA4350.15085519288657076No Hit
GTTATGAGTACGACCAAGTGCAAAAATTATTCATTCCCTTGGATTTTCAAGGATCGTCGAGGACGCACCGGACCC4340.15050839933970508No Hit
CTTCTGTCGGGTCCGGTGCGTCCTCGACGATCCTTGAAAATCCAAGGGAATGAATAATTTTTGCACTTGGTCGTA4220.14634687677731695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.1401045929337347No Hit
CACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTC4010.13906421229313765No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGT4000.13871741874627197No Hit
TCCACCAACAGCGCCTGGCTAGATCCACAAGAGTTGCCCCAAGCAGTCCCAGCTGAGCACCGAGGCTTTCTACCG3990.1383706251994063No Hit
GGTAACAGCAGTTGGACATGGGTCAGTCGATCCTAAGAGATAGGGAAGCTCCGTTTCAAAGTGCACGATTCTTCG3920.13594307037134654No Hit
AACTTGGAGACATCGGCGGGGGCCCCGGGAAGAGTTATCTTTTCTCCTTAACAGTCTACAACCTTGAAATCGGTT3910.13559627682448086No Hit
GAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTCTGTCGGGTCCGGTGCGTCCTCGACGATCCTTGAAAATC3890.13490268973074948No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA3840.1331687219964211No Hit
GTTTCAAAGTGCACGATTCTTCGTGCCGCCTATCGAAAGGGAATCCGGTTAATATTCCGGAACCAAGATGCAGAT3810.13212834135582405No Hit
GCTTGGGGCAACTCTTGTGGATCTAGCCAGGCGCTGTTGGTGGATGACCTTGGCTGCCCTCTCGGGGGCGTCTGG3780.13108796071522702No Hit
GTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATCCACCAACAGCGCCTGGCTAGATCCACA3750.13004758007462996No Hit
ACCCATACCTCGCCGTCAGCGTTGAAGTGACGCGCTGACGAGTAGGCAGGCGTGGAGGTCGGTGAAGAAGCCTTG3710.12866040588716726No Hit
TGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG3700.12831361234030156No Hit
CGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATCCACCAACAGCGCCTGGCTAGATCCACAAGAGTTGCCCCAA3700.12831361234030156No Hit
GTACGACCAAGTGCAAAAATTATTCATTCCCTTGGATTTTCAAGGATCGTCGAGGACGCACCGGACCCGACAGAA3660.12692643815283886No Hit
ACGCTGGACCGTGTACAAGTCTCCTGGAACGGAGCGTCGTAGAGGGTGAGAATCCCGTATATGACACGGACTACC3590.1244988833247791No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTCGGGCC3580.12415208977791341No Hit
GCGTACGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATCCACCAACAGCGCCTGGCTAGATCCACAAGAGTTGC3560.12345850268418206No Hit
GTGCACGATTCTTCGTGCCGCCTATCGAAAGGGAATCCGGTTAATATTCCGGAACCAAGATGCAGATTTTTAACG3540.12276491559045069No Hit
TCCACCAACAGCGCCTGGCTAGATCCACAAGAGTTGCCCCAAGCAGTCCCAGCTGAGCACCGCGGCTTTCTACCG3540.12276491559045069No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGCCG3520.12207132849671933No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA3510.12172453494985365No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAAAAATT3500.12137774140298797No Hit
ATCGAAAGGGAATCCGGTTAATATTCCGGAACCAAGATGCAGATTTTTAACGGCAACGTAAATGAACTTGGAGAC3480.12068415430925661No Hit
GTCCGGAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTCTGTCGGGTCCGGTGCGTCCTCGACGATCCTTGA3450.11964377366865957No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA3440.11929698012179389No Hit
CTCTTAGGATCGACTGACCCATGTCCAACTGCTGTTACCATGGAACCTTTCTCCACTTCAGTCTTCAAGGTTCTC3390.11756301238746548No Hit
GCCCCAAGCAGTCCCAGCTGAGCACCGCGGCTTTCTACCGAGGCCCGACGCACCCAACCCTTAGAGCCAATCCTT3370.11686942529373413No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT3300.11444187046567436No Hit
GCTGAGCACCGCGGCTTTCTACCGAGGCCCGACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGA3290.11409507691880869No Hit
CCTTAACAGTCTACAACCTTGAAATCGGTTTGTCCGGAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTCTG3240.11236110918448029No Hit
GCCCCAAGCAGTCCCAGCTGAGCACCGAGGCTTTCTACCGAGGCCCGACGCACCCAACCCTTAGAGCCAATCCTT3200.11097393499701756No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT3160.10958676080955485No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT3150.10923996726268917No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG3130.1085463801689578No Hit
GGTTAATATTCCGGAACCAAGATGCAGATTTTTAACGGCAACGTAAATGAACTTGGAGACATCGGCGGGGGCCCC3100.10750599952836079No Hit
TCCCTATCTCTTAGGATCGACTGACCCATGTCCAACTGCTGTTACCATGGAACCTTTCTCCACTTCAGTCTTCAA3030.10507844470030103No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT3020.10473165115343534No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGAC2990.1036912705128383No Hit
CTTGTGGATCTAGCCAGGCGCTGTTGGTGGATGACCTTGGCTGCCCTCTCGGGGGCGTCTGGCGTACGCTTAACA2990.1036912705128383No Hit
GGTCCGGTGCGTCCTCGACGATCCTTGAAAATCCAAGGGAATGAATAATTTTTGCACTTGGTCGTACTCATAACC2960.10265088987224126No Hit
GCTCCGGACAAACCGATTTCAAGGTTGTAGACTGTTAAGGAGAAAAGATAACTCTTCCCGGGGCCCCCGCCGATG2930.10161050923164422No Hit
GTTGGTGGATGACCTTGGCTGCCCTCTCGGGGGCGTCTGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGA2920.10126371568477854No Hit
CTCCAAGTTCATTTACGTTGCCGTTAAAAATCTGCATCTTGGTTCCGGAATATTAACCGGATTCCCTTTCGATAG2900.10057012859104719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)