FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288356
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAACCTTGAAATCGGTTTGTCCGGAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTCTGTCGGGTCC16870.585040713562402No Hit
GTTGTAGACTGTTAAGGAGAAAAGATAACTCTTCCCGGGGCCCCCGCCGATGTCTCCAAGTTCATTTACGTTGCC12890.44701688190986144No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTCG11550.4005465466298603No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAA9450.32771990178806754No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT9280.32182441149135094No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAAAAATTATTCATTCCCTTGG8830.30621870188239536No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATCCACCAACAGCGCCTGG8340.2892258180859771No Hit
ATCCTAAGAGATAGGGAAGCTCCGTTTCAAAGTGCACGATTCTTCGTGCCGCCTATCGAAAGGGAATCCGGTTAA8310.28818543744538No Hit
CTCCTTAACAGTCTACAACCTTGAAATCGGTTTGTCCGGAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTC7200.24969135374328955No Hit
GGCTGGTAGAGCTCGACACTTCTGTCGGGTCCGGTGCGTCCTCGACGATCCTTGAAAATCCAAGGGAATGAATAA7020.2434490698997073No Hit
CAGCTGGGACTGCTTGGGGCAACTCTTGTGGATCTAGCCAGGCGCTGTTGGTGGATGACCTTGGCTGCCCTCTCG6970.24171510216537892No Hit
GTCCCAGCTGAGCACCGAGGCTTTCTACCGAGGCCCGACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT6240.21639917324418426No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC6230.21605237969731858No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT6100.21154406358806477No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5890.20426139910388547No Hit
GTCCCAGCTGAGCACCGCGGCTTTCTACCGAGGCCCGACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT5880.20391460555701982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.19767232171343757No Hit
GTCCTCGACGATCCTTGAAAATCCAAGGGAATGAATAATTTTTGCACTTGGTCGTACTCATAACCGCAGCAGGTC5640.1955915604322435No Hit
CTACCAGCCATTGAACCTTAGCTCCGGACAAACCGATTTCAAGGTTGTAGACTGTTAAGGAGAAAAGATAACTCT5600.19420438624478076No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATCCACCAA5490.1903896572292583No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT5440.1886556894949299No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAAAAATTATTCA5280.183106992745079No Hit
GTTAAGGAGAAAAGATAACTCTTCCCGGGGCCCCCGCCGATGTCTCCAAGTTCATTTACGTTGCCGTTAAAAATC5210.18067943791701924No Hit
GAGTACGACCAAGTGCAAAAATTATTCATTCCCTTGGATTTTCAAGGATCGTCGAGGACGCACCGGACCCGACAG5060.17547753471403404No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG4990.1730499798859743No Hit
GTCGAGGACGCACCGGACCCGACAGAAGTGTCGAGCTCTACCAGCCATTGAACCTTAGCTCCGGACAAACCGATT4720.16368655412060093No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCAAGGTCATC4640.16091220574567547No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCTATATTTAGCTTTAGATGGAATTTACCACCCATTTTGAGCTGCATTCC4610.15987182510507844No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAAAAATTATTCATTCCCTTGGATTTTCAAGGATCGT4560.15813785737075003No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG4390.15224236707403346No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC4310.14946801869910806No Hit
GTCCAGCGTGGGTAACACTTCTCTAAATTACAACTCGGACGGCCTCGGGGACCGCCAGATTTTAAATTTGAGCTT4300.14912122515224238No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA4250.14738725741791397No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA4240.1470404638710483No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT4110.14253214776179446No Hit
AGATAGGGAAGCTCCGTTTCAAAGTGCACGATTCTTCGTGCCGCCTATCGAAAGGGAATCCGGTTAATATTCCGG4080.1414917671211974No Hit
TGATTGGACCGCTCGGCGATTGAGAGTTTCTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAAC4010.13906421229313765No Hit
ACGCTGGACCGTGTACAAGTCTCCTGGAACGGAGCGTCGTAGAGGGTGAGAATCCCGTATATGACACGGACTACC4000.13871741874627197No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCC3940.1366366574650779No Hit
GTGCTCAGCTGGGACTGCTTGGGGCAACTCTTGTGGATCTAGCCAGGCGCTGTTGGTGGATGACCTTGGCTGCCC3800.13178154780895837No Hit
CTTCTGTCGGGTCCGGTGCGTCCTCGACGATCCTTGAAAATCCAAGGGAATGAATAATTTTTGCACTTGGTCGTA3670.12727323169970453No Hit
GAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTCTGTCGGGTCCGGTGCGTCCTCGACGATCCTTGAAAATC3630.1258860575122418No Hit
GTCCGGAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTCTGTCGGGTCCGGTGCGTCCTCGACGATCCTTGA3580.12415208977791341No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA3560.12345850268418206No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA3540.12276491559045069No Hit
GTTATGAGTACGACCAAGTGCAAAAATTATTCATTCCCTTGGATTTTCAAGGATCGTCGAGGACGCACCGGACCC3510.12172453494985365No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGT3480.12068415430925661No Hit
CACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTC3470.12033736076239093No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGCCG3460.11999056721552524No Hit
GCTTGGGGCAACTCTTGTGGATCTAGCCAGGCGCTGTTGGTGGATGACCTTGGCTGCCCTCTCGGGGGCGTCTGG3380.11721621884059981No Hit
TGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG3330.1154822511062714No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTCGGGCC3320.11513545755940573No Hit
TCCACCAACAGCGCCTGGCTAGATCCACAAGAGTTGCCCCAAGCAGTCCCAGCTGAGCACCGCGGCTTTCTACCG3310.11478866401254005No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAAGTGCAAAAATT3250.11270790273134597No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA3190.11062714145015189No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA3170.10993355435642052No Hit
CCTTAACAGTCTACAACCTTGAAATCGGTTTGTCCGGAGCTAAGGTTCAATGGCTGGTAGAGCTCGACACTTCTG3140.10889317371582348No Hit
GGTAACAGCAGTTGGACATGGGTCAGTCGATCCTAAGAGATAGGGAAGCTCCGTTTCAAAGTGCACGATTCTTCG3120.10819958662209213No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCAGACGCCCCCGAGAGGGCAGCCA3110.10785279307522644No Hit
ACCCATACCTCGCCGTCAGCGTTGAAGTGACGCGCTGACGAGTAGGCAGGCGTGGAGGTCGGTGAAGAAGCCTTG3110.10785279307522644No Hit
AACTTGGAGACATCGGCGGGGGCCCCGGGAAGAGTTATCTTTTCTCCTTAACAGTCTACAACCTTGAAATCGGTT3090.1071592059814951No Hit
TCCACCAACAGCGCCTGGCTAGATCCACAAGAGTTGCCCCAAGCAGTCCCAGCTGAGCACCGAGGCTTTCTACCG3060.10611882534089806No Hit
GTTTCAAAGTGCACGATTCTTCGTGCCGCCTATCGAAAGGGAATCCGGTTAATATTCCGGAACCAAGATGCAGAT3050.10577203179403238No Hit
GTACGACCAAGTGCAAAAATTATTCATTCCCTTGGATTTTCAAGGATCGTCGAGGACGCACCGGACCCGACAGAA3020.10473165115343534No Hit
CTCTTAGGATCGACTGACCCATGTCCAACTGCTGTTACCATGGAACCTTTCTCCACTTCAGTCTTCAAGGTTCTC2990.1036912705128383No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG2930.10161050923164422No Hit
CTCCAAGTTCATTTACGTTGCCGTTAAAAATCTGCATCTTGGTTCCGGAATATTAACCGGATTCCCTTTCGATAG2920.10126371568477854No Hit
GCTGAGCACCGCGGCTTTCTACCGAGGCCCGACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGA2910.10091692213791287No Hit
ATCGAAAGGGAATCCGGTTAATATTCCGGAACCAAGATGCAGATTTTTAACGGCAACGTAAATGAACTTGGAGAC2890.1002233350441815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)