FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195602
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28851.4749337941329843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.9590903978486929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.5449842026155152No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.4325109150213188No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.2837394300671772No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCAC5500.2811832189854909No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT4910.25101992822159286No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.2162554575106594No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTC4230.2162554575106594No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCA3380.17279986912199263No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.17279986912199263No Hit
GTCTGTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCA2910.14877148495414158No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.14774900052146706No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT2840.14519278943978078No Hit
GTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCACGGT2820.14417030500710626No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA2670.13650167176204742RNA PCR Primer, Index 27 (96% over 29bp)
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT2580.13190049181501212No Hit
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA2570.13138924959867485No Hit
ATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCC2390.12218688970460426No Hit
GATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAG2160.11042831872884736No Hit
GTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCG2080.10633838099814931No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTT2040.10429341213280027No Hit
TTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC2020.10327092770012576No Hit
ATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCG1960.10020347440210223No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)