FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689150
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG138412.008416164840746Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG114121.6559529855619242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT57050.8278313864906043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC47270.6859174345207865Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG43630.6330987448305885Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG36010.522527751578031Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT35160.5101937168976275Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA33940.4924907494739897Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC30990.44968439381847203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG24980.36247551331350214Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23100.33519553072625696Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC22680.3291010665312341Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22390.3248929841108612Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT20690.3002249147500544Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT20070.2912283247478778Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC17420.2527751578030908Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGATCCGCTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCT16620.24116665457447584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.2077922077922078No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC14070.20416455053326563Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG13990.2030037002104041Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC13530.19632881085395051Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG12580.18254371326997026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCTAAACCGTATGTGTGTGATACCCGGCAGGGGTTGCATGTGCGGTGTTGTGGGCCCCTCCTTGTCATGTCTG12120.17586882391351666No Hit
GATCAGTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAACCATGTGCCTACA11820.17151563520278604No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT11340.16455053326561705Clontech SMART CDS Primer II A (96% over 26bp)
GGTCTAGGACACGCGACTCAATCGCCCTATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGC10700.15526373068272512No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10510.15250671116592904Clontech Universal Primer Mix Long (96% over 26bp)
ATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTC10430.15134586084306756No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC9860.14307480229267938Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG9690.1406079953565987Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9640.13988246390481027Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA9470.1374156569687296Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG9450.13712544438801422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGTGGGCCCCTCCTTGTCATGTCTGCCGGCATGGCGCAGTGAGGTGCGGGCATATAGACGAACCAGTGTGGAT9110.13219183051585287No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9050.13132119277370674Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA9000.13059566132191833Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.12783864180512225No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT8240.1195675832547341Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA8000.1160850322861496Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT7920.11492418196328812Clontech Universal Primer Mix Long (96% over 26bp)
CACATAGACCGGGTGGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAGGAAGAGAAAACAAAAGTGATT7870.11419865051149967No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT7840.11376333164042661Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT7800.11318290647899586Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC7660.11115141841398825Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTACTCGGGCAACCCTCCAACAGCGGCGGAACACATTTCGACTACGGGACTCTCACCCTCTACGGTCCAGCA7650.11100631212363056No Hit
CATATAGACGAACCAGTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCGTAGTCGAAATGTGTTCCGCCGCTGT7430.10781397373576145No Hit
GGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCCACAAGGAGGCTC7260.10534716679968077No Hit
GCGCAGTGAGGTGCGGGCATATAGACGAACCAGTGTGGATGCTGGACCGTAGAGGGTGAGAGTCCCGTAGTCGAA7250.10520206050932308No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAGAT7040.10215482841181166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCTCGGGCCCCGTGCTACTCGGGCAACCCTC7040.10215482841181166No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAGAT6940.10070376550823479Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCT6940.10070376550823479No Hit
GTCTATGTGTTCAACCGGGGGTCACACGACACGTCGTGCGGGGTTTCCCCATTCGGAAATCCTGGTCTCAACGTC6920.10041355292751941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)