FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410726
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80961.971143779551331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48901.1905747383900702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33570.8173332099745328No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGAGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22100.5380716097836514RNA PCR Primer, Index 26 (96% over 28bp)
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC21270.5178634905021839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.4952206580542746No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA14960.3642330896997025No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC14320.34865092543447457No Hit
TATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTG13170.32065172402039316No Hit
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG12630.30750427292160715No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA11100.27025316147504663No Hit
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT10430.25394058325988617No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.2480972716604257No Hit
TACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAA9390.22861956632889077No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT8660.2108461602138652No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.2059767338809815No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.18576861459951405No Hit
GTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGG7280.17724711851696753No Hit
ATACACATCTCCGAGCCCACGAGACTAGAGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7130.17359504876730472RNA PCR Primer, Index 26 (96% over 28bp)
GTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCG6550.15947371240194191No Hit
GGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAG6420.15630858528556751No Hit
TTATACACATCTCCGAGCCCACGAGACTAGAGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA6320.15387387211912565RNA PCR Primer, Index 19 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.14876097446959774No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT5700.1387786504871861No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA5570.1356135233708117No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC5510.13415269547094658No Hit
ATTTAATGCCATGCTCTACCAATTTATTACACCATATCTCTCTATGAAAGACTTCCATGGTGAGTACAACTGTAA5480.13342228152101399No Hit
CAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGC5460.13293533888772563No Hit
CTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTC5450.13269186757108145No Hit
GTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTC5410.1317179823045047No Hit
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG5370.13074409703792794No Hit
GCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAG5190.1263616133383326No Hit
TTATTACACCATATCTCTCTATGAAAGACTTCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGA5030.12246607227202563No Hit
ATTTATAGCTGCTCACAGAAAGCAGGTGGTTATCACATAAGATCCGCTTAGGCTTTTTCTGCTGTAGCTTCAGTT5000.12173565832209307No Hit
ATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTA4910.1195444164722954No Hit
AGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGC4810.11710970330585353No Hit
GGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCT4790.11662276067256516No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACTTCCCTTACCTACATTGATCTATCGACT4780.11637928935592098No Hit
GATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGAT4770.11613581803927679No Hit
GTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAA4720.11491846145605586No Hit
ATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTT4510.10980556380652795No Hit
GTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTC4460.10858820722330703No Hit
CTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCC4360.10615349405686517No Hit
GGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAA4320.10517960879028843No Hit
GGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAA4310.10493613747364423No Hit
CTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGA4270.1039622522070675No Hit
CTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTCTC4240.10323183825713493No Hit
CTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCA4230.10298836694049075No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)