FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410726
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30640.7459961141977863No Hit
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC17320.4216923204277304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.41463165224504894No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC13270.32308643718683505No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA12560.3057999737050978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.27512258780793036No Hit
TATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTG11220.2731748172747769No Hit
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG10500.25564488247639544No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA9990.24322784532754196No Hit
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT9310.2266717957957373No Hit
TACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAA8700.21182004548044195No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT7670.1867424998660908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.1777340611502559No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT6130.14924791710288612No Hit
GGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAG6120.14900444578624192No Hit
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG5800.14121336365362797No Hit
ATTTAATGCCATGCTCTACCAATTTATTACACCATATCTCTCTATGAAAGACTTCCATGGTGAGTACAACTGTAA5180.12611814202168842No Hit
GTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCG5150.12538772807175586No Hit
GTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGG5080.12368342885524655No Hit
CTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAGTCTGTACCGAATCCGCAGCAGGTC5080.12368342885524655No Hit
GTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTC5050.122953014905314No Hit
AGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGC4940.12027483042222796No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC4910.1195444164722954No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACTTCCCTTACCTACATTGATCTATCGACT4760.1158923467226326No Hit
GCATTAAATTGCTGTGTCGATATTCTCTTATTGGACCTTGAAAATTCATGAAAGGGCCACGCAAATTCTCAACAG4690.1141880475061233No Hit
CAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGC4690.1141880475061233No Hit
ATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTA4640.11297069092290238No Hit
ATTTATAGCTGCTCACAGAAAGCAGGTGGTTATCACATAAGATCCGCTTAGGCTTTTTCTGCTGTAGCTTCAGTT4610.11224027697296982No Hit
TTATTACACCATATCTCTCTATGAAAGACTTCCATGGTGAGTACAACTGTAAAACAGAAAAGAAAACTCTTCCGA4560.11102292038974888No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA4480.1090751498565954No Hit
CTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAATTTGCGTGGCCCTTTCATGAATTTTCA4410.1073708506400861No Hit
GTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTC4400.10712737932344191No Hit
GGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCT4280.10420572352371167No Hit
GTTATACACTTTGGTTGGGCTGGCAACGGCCCTTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAAC4250.10347530957377912No Hit
GATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGAT4160.10128406772398145No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)