FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213663
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51772.422974497222261No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGAGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA37551.757440455296425RNA PCR Primer, Index 26 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29001.3572775819865863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.8883147760726002No Hit
ATACACATCTCCGAGCCCACGAGACTAGAGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA13250.6201354469421472RNA PCR Primer, Index 26 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.5981381895789163No Hit
TTATACACATCTCCGAGCCCACGAGACTAGAGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA9770.45726213710375685RNA PCR Primer, Index 19 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.38471799048033584No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.2925167202557298No Hit
ACACATCTCCGAGCCCACGAGACTAGAGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA5020.2349494297094022RNA PCR Primer, Index 26 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.2115480920889438No Hit
TATACACATCTCCGAGCCCACGAGACTAGAGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3520.16474541684802704RNA PCR Primer, Index 26 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.14321618623720533No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGAGAGAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA2150.10062575176797105TruSeq Adapter, Index 8 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)