FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128104
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29932.3363829388621746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17771.3871541872228814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14091.0998875913320427No Hit
CAGCTTAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCT13181.0288515581090365TruSeq Adapter, Index 2 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.6939674014862924No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA8460.6604009242490476TruSeq Adapter, Index 2 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.2880472116405421No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.279460438393805No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.23496534066071317No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.22247548866545933No Hit
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.215449946918129No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2490.19437332167613813RNA PCR Primer, Index 32 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA2130.16627115468681697TruSeq Adapter, Index 2 (96% over 27bp)
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.13036283020046213No Hit
CAGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.1288015987010554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1540.12021482545431837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1390.10850558920876788No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.10148004746143757No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG1290.10069943171173422No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)