FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128104
Sequences flagged as poor quality0
Sequence length75
%GC34

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTAAGCTGCTGTCTCTTATACACATCTGACGCTGCCGACGAAGTTAACAGTGTAGATCTCGGTGGTCGCCGT13031.0171423218634859Illumina Single End PCR Primer 1 (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.9976269281209017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.5370636357959158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.4246549678386311No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.2412102666583401No Hit

[FAIL]Adapter Content

Adapter graph