FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002147594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093790
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC126531.1568034083324952No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118311.0816518710172884No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG105080.9606962945355142No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG73580.6727068267217656No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG69220.6328454273672277No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT53030.48482798343374867No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGT46660.42659011327585733No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC40800.3730149297397124No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC36320.3320564276506459No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT35820.32748516625677687No Hit
GATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGC35430.3239195823695591No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT33600.30718876566799846No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG32900.3007889997165818No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG29140.26641311403468676No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT27000.2468481152689273No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT26400.24136260159628448No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC26210.23962552226661424No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG24710.22591173808500717No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT24500.2239918082995822No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT22620.20680386545863463No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG22600.2066210150028799No Hit
ATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT22410.20488393567320967No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22060.20168405269750134Clontech Universal Primer Mix Long (96% over 26bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC21580.1972956417593871No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA21240.19418718401155616No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT18210.16648533996470985No Hit
TGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCC18020.16474826063503964No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17680.1616398028872087No Hit
CTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCG17220.15743424240484918No Hit
GTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC16980.15524003693579205No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA16840.15396008374550874No Hit
TAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTG16540.15121732690918732No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA16290.1489316962122528No Hit
CCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACG16090.1471031916547052No Hit
ACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG15660.14317190685597783No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC15500.14170910320993974No Hit
GGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACA14410.13174375337130528No Hit
CACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTA14380.13146947768767314No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC14230.13009809926951243No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG14100.12890957130710648No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG14010.12808674425621006No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG13910.12717249197743624No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT13800.12616681447078507No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGT13530.1236983333180958No Hit
AATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG12460.1139158339352161No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC12250.1119959041497911No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA11910.10888744640196014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT11870.10852174549045063No Hit
TGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCC11570.10577898865412921No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC11400.10422475978021375No Hit
TATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA11360.10385905886870422No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC11270.1030362318178078No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA11110.10157342817176973No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG11070.1012077272602602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)