FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002147617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847791
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC172590.9340342062495164No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG169380.9166621116782147No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG116730.6317272895040619No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC94110.5093108473847963No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT86680.4691006720998208No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGT80530.4358176871734952No Hit
GATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGC64030.34652187395652434No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG56020.3031728155402857No Hit
CACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTA55460.3001421697583764No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT54480.29483853964003504No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT54460.29473030229068115No Hit
GATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAAT46420.25121888785041163No Hit
ACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATAT46350.250840057127673No Hit
ATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT42890.23211499568944757No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG42430.22962553665430777No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC42330.22908434990753823No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG40500.219180632441656No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA38590.20884396557835816No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT38490.20830277883158865No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT37700.2040274035321094No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36480.19742492522152127No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG36300.19645078907733612No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT36240.19612607702927443No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC33910.18351642582954458No Hit
CTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCG32680.17685982884427948No Hit
TGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCC28870.1562406137923607No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA28120.1521817131915893No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG27410.1483392872895257No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG26900.14557923488100116No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC26830.1452004041582625No Hit
TTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCC26200.1417909276536145No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT25440.13767790837816613No Hit
TGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT25180.1362708228365654No Hit
CCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACG24980.13518844934302635No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC24750.13394371982545644No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC24100.13042600597145457No Hit
GGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACA24060.13020953127274676No Hit
AATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG24040.13010129392339284No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA23250.12582591862391362No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT23010.12452707043166679No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT23000.12447295175698983No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT22400.12122583127637271No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC21710.11749164272366303No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGT21660.11722104935027826No Hit
ACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG21310.11532689573658492No Hit
TGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCC20830.11272919935209123No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC20670.11186330055725999No Hit
TTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTAC20360.11018562164227448No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT20100.10877853610067373No Hit
GTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC19650.10634319574021088No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG19550.10580200899344136No Hit
ATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAA19220.10401609272910194No Hit
AAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTA18640.10087720959783872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)