FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002147629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609668
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269841.6763705310660335No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC224141.3924610540807172No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG144230.8960232793346204No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA132610.8238344801536713No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC116250.7221986148696501No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT111230.6910120596296876No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT101210.6287631983738262No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA92080.5720434275887947No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC79750.49544378095358793No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG75630.4698484407964872No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT67000.41623490061304563No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT66240.411513430098629No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG63990.3975353923914745No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA59840.3717536783982784No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT59440.36926869391700645No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT58120.36106824512880914No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT54730.3400080016500297No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG54170.336529023376249No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC52330.3250980947623982No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA52060.32342073023753964No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA51250.318388636662964No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT49410.30695770804911326No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG49300.30627433731676346No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG48580.301801365250474No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC47840.29720414396012096No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA47650.29602377633151683No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG47540.295340405599167No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC47000.29198567654944996No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT44130.27415591289632396No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT43630.2710496822947341No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT37680.23408553813581437No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA37440.2325945474470512No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC37170.23091718292219265No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC34980.21731189288722894No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG34970.2172497682751971No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC34890.21675277137894275No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA34270.21290104543297125No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC33520.20824169953058644No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG33380.20737195496214125No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT31980.1986745092776896No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT31170.19364241570311394No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG29710.18457222234647144No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC29500.1832676054938037No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG29330.18221148708926313No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT28910.1796022533839276No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG27370.17003506313103073No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG26420.16413322498800995No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT25830.16046787287813385No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACC25350.15748589150060757No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA25330.15736164227654398No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT25110.15599490081184442No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC24960.15506303163136745No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC24830.15425541167495407No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA24820.1541932870629223No Hit
CCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCG24190.15027943650491904No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT23930.1486641965920923No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC23890.14841569814396507No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG23770.1476702027995835No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC23640.14686258284317014No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA23230.14431547374986642No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG23160.14388060146564385No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTA22840.14189261388062632No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT22630.14058799702795857No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG22560.14015312474373598No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA22440.13940762939935442No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC22260.13828938638278204No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC22010.13673627108198708No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA21350.13263604668788842No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG21260.13207692517960223No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA21030.13064805910287092No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG20950.1301510622066165No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACG20930.13002681298255292No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC20820.12934344225020314No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC20610.1280388253975354No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT20530.127541828501281No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCG20180.12536746708016808No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA19900.12362797794327775No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG19570.1215778657462284No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCC19530.12132936729810123No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG18200.11306679389787211No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG18120.11256979700161773No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG17970.11163792782114075No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA17930.11138942937301358No Hit
GGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGA17840.11083030786472738No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA17380.10797257571126467No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGT17330.1076619526511057No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG17180.10673008347062873No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATG16660.10349960364497524No Hit
AATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAT16650.10343747903294342No Hit
GTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGG16630.10331322980887984No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGC16590.10306473136075264No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC16540.10275410830059366No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT16280.10113886838776691No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC16140.10026912381932175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)