FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158997
Sequences flagged as poor quality0
Sequence length20-100
%GC27

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123697.7793920640012075No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46382.917036170493783No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27171.7088372736592514No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19721.240274973741643No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15240.9585086511066246No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12190.7666811323484091No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11300.7107052334320774No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6610.4157311144235426No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6320.39749177657440077No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4230.2660427555236891No Hit
ATGGGAAGCAGTGGTATCAACGC4090.25723755794134484No Hit
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.23585350666993718No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3660.23019302250985868Clontech Universal Primer Mix Long (96% over 26bp)
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3510.22075888224306117No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3310.20818002855399786No Hit
ATACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.19182751875821558No Hit
ATGGGGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.1786197223846991No Hit
ATACATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.1729592382246206No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2680.16855663943344842No Hit
ATGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.16226721258891677No Hit
ATGGATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.12956219299735214No Hit
ATGGGAAGCAGTGGTATCAAC2050.12893325031289898No Hit
ATACATGGATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.12515959420618No Hit
ATACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.12453065152172682No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1940.12201488078391416No Hit
ATGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.11698333930828884No Hit
ATGGGAAGCAGTAGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.113838625886023No Hit
ATACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.11195179783266351No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1770.11132285514821035Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGCAGTGGTATCAACGC1720.1081781417259445No Hit
ATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.10629131367258503No Hit
ATACATGGAGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.10377554293477237No Hit
ATAACAACACAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1630.10251765756586603No Hit
ATACATGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1620.10188871488141285No Hit
ATGGGAAGCGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.1012597721969597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)