FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210714
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCAC14370.681967026395968No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCA9740.4622379148988677No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTC8910.422848030980381No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT8880.42142430023633926No Hit
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA7760.36827168579211633No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT7610.3611530320719079No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT7560.35878014749850506No Hit
GTGTACACGTGTTCGTTGAGCAAAGATGCAAATTTAAGCTTTCTCCTCAGCTGAGACAGCAGTGGCGACTGATTG7540.35783099366914395No Hit
CGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTG7530.3573564167544634No Hit
GTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAA7520.35688183983978283No Hit
GTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCG7500.3559326860104217No Hit
CTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGTCT6760.3208139943240601No Hit
ATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCC6570.31179703294512945No Hit
TGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATG5850.27762749508812895No Hit
CTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCGCAGCAGGTCTC5480.26006814924494814No Hit
GTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGG5300.25152576478069805No Hit
GTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAAT4940.23444099585219774No Hit
CACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAG4890.23206811127879493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.20074603490987786No Hit
GTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATAGATTTTCAAGGTCCAAGGAGAGGGTACCGACACAGCAAT4160.1974239965071139No Hit
TAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCAC4010.19030534278690547No Hit
CCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAG3950.1874578812988221No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTT3920.1860341505547804No Hit
GATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAG3870.18366126598137758No Hit
GGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTC3860.18318668906669705No Hit
CTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTA3820.1812883814079748No Hit
TTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC3790.17986465066393312No Hit
GCCCAATGATGGGTATTTCTTTTGAAGAGCAGCACGATATTTAGGATGGCTGATACCGTAAACGATTGGATTGTA3770.17891549683457197No Hit
TTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGAC3750.17796634300521086No Hit
GCTCTGAAGACTGTGGCAGCTCGGGCTTTACAATATGCATTCGCTTCACGGTGAATGTATATTAAGTCTGTAATG3710.1760680353464886No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3600.1708476892850024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTG3580.1698985354556413No Hit
GTCTGTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCA3520.16705107396755792No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAA3470.16467818939415513No Hit
ATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCG3460.16420361247947454No Hit
AAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAAC3430.16277988173543287No Hit
GTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGGAAG3420.1623053048207523No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3390.1608815740767106Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCAT3280.15566122801522442No Hit
CTGTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGA3220.15281376652714107No Hit
GTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGATACCTCCCATTGGCTTCTCTATGAGAGCTTCTCGTT3220.15281376652714107No Hit
CTCAATAGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAG3200.15186461269777993No Hit
GTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCT3170.15044088195373825No Hit
GTCCAGAGAGCAGCCCAAACCCAGATGGCAGCAATTTTTCCCATGGCACCTCCGCTTGTCAATGGCTTGCCTGCG3150.1494917281243771No Hit
GTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGATA3100.14711884355097432No Hit
AGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATT3070.14569511280693262No Hit
CCTTGCGACACACGCACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCT3050.1447459589775715No Hit
GTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCACGGT3050.1447459589775715No Hit
TCTCTGGACTGGTGCTCCTTTCTTCGGCTGGAACAGATATGTGCCAGAAGGAAACATGACCGCTTGCGGAACTGA3030.14379680514821036No Hit
GCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAG3020.1433222282335298No Hit
GAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGC2990.14189849748948813No Hit
GTCCAAGGAGAGGGTACCGACACAGCAATTTAATGCCATGCTCTACCAATCTGTTATACCATATCTCTCTATGAA2880.13667815142800194No Hit
ATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACT2770.13145780536651575No Hit
CGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACT2750.13050865153715463No Hit
GATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACAC2700.1281357669637518No Hit
ATCGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTG2690.12766119004907125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.1271866131343907No Hit
GTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCAC2630.12481372856098787No Hit
CTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATAGATTTTCA2620.12433915164630732No Hit
GGCTGATACCGTAAACGATTGGATTGTAGATGGCGTTGGCCTTGGCGAAGACTGAGCCCCAGATGGTGGCGAGTG2580.12244084398758508No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAG2570.1219662670729045No Hit
CTATGGGACCACCAATGGTTATTAACTGCTATCACGGAACATGGACTTTCGGCGCTGCTGCTTGCCAGCTTTATG2540.12054253632886282No Hit
GAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACTAGAGA2540.12054253632886282No Hit
CCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACC2450.11627134409673776No Hit
GTATATTAAGTCTGTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCA2430.11532219026737663No Hit
GTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCG2410.11437303643801551No Hit
CTGTAATGGTGCGTGTGTCGCAAGGCGTGCGTGCCTAAAAGGTTGTCAATTAACAGTCAATTCAGAACTGGCACG2400.11389845952333494No Hit
CTCTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGT2380.11294930569397382No Hit
ACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGC2380.11294930569397382No Hit
GTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCA2310.10962726729120988No Hit
ATTACAGACTTAATATACATTCACCGTAAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCC2300.10915269037652933No Hit
GTACTGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGG2300.10915269037652933No Hit
GTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGAC2290.10867811346184876No Hit
AGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTCGGGC2290.10867811346184876No Hit
ACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATA2270.10772895963248763No Hit
CCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGA2250.10677980580312652No Hit
GCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGA2210.10488149814440427No Hit
GTTATGCATGAGTCGGATAATCTTTCGTGAATAGTCGTTTAATGTTATGATAACTCGTTAAAGTTATCGTTTATG2210.10488149814440427No Hit
ATATTTAGGATGGCTGATACCGTAAACGATTGGATTGTAGATGGCGTTGGCCTTGGCGAAGACTGAGCCCCAGAT2160.10250861357100145No Hit
GGGTACCGACACAGCAATTTAATGCCATGCTCTACCAATCTGTTATACCATATCTCTCTATGAAAGACTTCCATG2160.10250861357100145No Hit
CCCTTACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTG2160.10250861357100145No Hit
GTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAATTTGCGTGTC2160.10250861357100145No Hit
GTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGA2140.10155945974164034No Hit
GACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCT2130.10108488282695977No Hit
GGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTCGGGCT2130.10108488282695977No Hit
GAGTATACGTTTGTCATTAGTCTCCATGTGTGATCGCAAGGTTGTGTATGGGTTAAAGGTTAATGACATCTCGGT2110.10013572899759864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)