FastQCFastQC Report
Wed 8 May 2024
EGAF00002147946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161325
Total Bases12 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30601.896792189679219No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23321.4455292112195877Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19651.218038121803812Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19191.1895242522857585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17871.1077018441035178No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10540.6533395320006199Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAG7730.47915698124903144No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7710.47791724779172473Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6980.432666976600031Clontech Universal Primer Mix Long (96% over 26bp)
GTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTG6790.4208895087556176No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6590.40849217418255074Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTG6480.40167364016736407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.399814039981404No Hit
GTTCCAGCCAGGTCCAAATTCTTCCAGCCAAGGCCCGATGCACCCAACCC6340.39299550596621724No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAG6210.38493723849372385No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6080.3768789710212304Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5920.366961103362777Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG5870.3638617697195103No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT5840.3620021695335503No Hit
GTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGC5530.3427863009452968No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGA5390.33410816674415No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5060.31365256469858976Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4820.29877576321090965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC4690.29071749573841627No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC4420.27398109406477605No Hit
GCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAG4340.26902216023554937No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGC3900.2417480241748024No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGA3730.23121028978769562No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT3700.2293506896017356No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAG3700.2293506896017356No Hit
GTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGG3680.22811095614442894No Hit
GTTATAGACGGTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAG3680.22811095614442894No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3640.2256314892298156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAAGGGCCCTGGGAAGAGTTATCTTTTCTCCTTGACCGTCTATAACCT3590.22253215558654887No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3570.22129242212924222Clontech Universal Primer Mix Long (100% over 23bp)
ATCCTAAGAGATAGGGAAGCTCCGTTTTAAAGTGCATGATTATCCATGCC3550.22005268867193556No Hit
TCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAGATCAGTTCCA3500.21695335502866886No Hit
ATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCC3440.2132341546567488No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3220.19959708662637532Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3190.19773748644041528Clontech Universal Primer Mix Long (96% over 26bp)
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA3020.18719975205330855No Hit
CCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTT2960.1834805516813885No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA2940.18224081822408184No Hit
GAGTAATACGTTCCGTCGGTTTTTCAAGGACTGTTAAGGACGCACCGGAT2910.18038121803812182No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG2900.17976135130946846No Hit
GTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAATGGATGGCGCT2890.17914148458081514No Hit
TCCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTTACTTA2890.17914148458081514No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT2830.17542228420889508No Hit
TGATTATCCATGCCACCCATCGAAAGGGAATCCGGTTAAGATTCCGGAAC2770.17170308383697505No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.16674415000774834No Hit
CTTCTGTTGCATCCGGTGCGTCCTTAACAGTCCTTGAAAAACCGACGGAA2660.1648845498217883No Hit
GATCAGTTCCAGCCAGGTCCAAATTCTTCCAGCCAAGGCCCGATGCACCC2650.16426468309313497No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT2610.16178521617852162No Hit
TCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCA2600.1611653494498683No Hit
GGCTGGAACTGATCTGGGCAACTGGGTTGGATCTGGCCGGGACTGAGTTT2600.1611653494498683No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT2500.1549666821633349No Hit
GTGCAGCACTTCTGTTGCATCCGGTGCGTCCTTAACAGTCCTTGAAAAAC2500.1549666821633349No Hit
GAGTACGACCAGGCATGAGAGTAATACGTTCCGTCGGTTTTTCAAGGACT2460.15248721524872152No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACATATATGCTCTTACT2450.1518673485200682No Hit
CCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCC2320.14380908104757475No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC2320.14380908104757475No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG2300.1425693475902681No Hit
GTGTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAATGGATGGCG2290.14194948086161474No Hit
ACCCATACCTCACCGTCAGCGTTTATGTGACGCGCTGACGAGTAGGCAGG2290.14194948086161474No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC2250.1394700139470014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT2240.13885014721834807No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.1382302804896947Clontech SMART CDS Primer II A (100% over 21bp)
CTCCTTGACCGTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTC2220.13761041376104136No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGAGAGTAATAC2160.13389121338912136No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAAT2160.13389121338912136No Hit
GTGCATGATTATCCATGCCACCCATCGAAAGGGAATCCGGTTAAGATTCC2150.133271346660468No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2150.133271346660468Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2140.13265147993181467Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT2140.13265147993181467Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTGGTCGTACTCA2140.13265147993181467No Hit
TCCCAGGGCCCTTACCAGCGTCACCAACTTCATTTACGTTACCGTCAAAG2130.1320316132031613No Hit
CATCAGACACCACAAAAGGTGTTAATTCATCTAGACAGCAGGACGGTGGC2110.13079187974585463No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACA2100.1301720130172013No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG2090.12955214628854797No Hit
GTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGC2070.12831241283124128No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGA2040.12645281264528127No Hit
TGCCTGAATAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGA2030.12583294591662794No Hit
GTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATTTC2030.12583294591662794No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1990.12335347900201457Clontech Universal Primer Mix Long (96% over 26bp)
GGTTAAGGATGTTTCATCCCCAAGGCCTCTAATCATTCGCTTTACCTCAT1950.1208740120874012No Hit
GTCCCGGCCAGATCCAACCCAGTTGCCCAGATCAGTTCCAGCCAGGTCCA1940.12025414535874787No Hit
CAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAAT1910.11839454517278784No Hit
CCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTGGT1860.11529521152952116No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1860.11529521152952116Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTAGGATCGACTAACCCATGTCCAACTGCTGTTTCCATGGAACCTTT1830.11343561134356114No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGA1830.11343561134356114No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAACTCG1810.11219587788625444No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAG1800.11157601115760112No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1790.11095614442894779Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1780.11033627770029443Clontech Universal Primer Mix Long (96% over 26bp)
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT1780.11033627770029443No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGT1750.10847667751433443No Hit
GCGTAGTACCCATACCTCACCGTCAGCGTTTATGTGACGCGCTGACGAGT1740.10785681078568107No Hit
GGGTTAGTCGATCCTAAGAGATAGGGAAGCTCCGTTTTAAAGTGCATGAT1730.10723694405702774No Hit
TTCAAGGACTGTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTG1720.1066170773283744No Hit
TCCCTATCTCTTAGGATCGACTAACCCATGTCCAACTGCTGTTTCCATGG1710.10599721059972106No Hit
GTCTAGATGAATTAACACCTTTTGTGGTGTCTGATGAGCGTAGATTCCGG1690.10475747714241439No Hit
GTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAGCGTCACCAAC1680.10413761041376103No Hit
CTGTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCAT1650.10227801022780103No Hit
GGTATGGGTACTACGCTTGAGCGCCATCCATTTTCAGGGCTAATTCATTC1630.10103827677049435No Hit
ATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTCTGTTGCAT1630.10103827677049435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)