FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161325
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20641.2794049279404929Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG16721.0364171703083838Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9300.5764760576476058Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGCCAGA7400.4587013792034712No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6650.41221137455447077Clontech Universal Primer Mix Long (96% over 26bp)
GTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATT6600.4091120409112041No Hit
GTCCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTC6290.38989617232295054No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6280.3892763055942972Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCAGCCAGGTCCAAATTCTTCCAGCCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGT6180.3830776383077638No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5870.3638617697195103Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT5480.3396869673020301No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.33906710057337675No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGAGAGTAATACGTTCCGTCGG5430.3365876336587634No Hit
GTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTCTGTTGCATCC5200.3223306988997366No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC5190.3217108321710832Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5180.3210909654424299Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTTGGGTGCATCG5100.31613203161320313No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGA4960.3074538974120564No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4280.26530295986362934Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCT4230.26220362622036264No Hit
GCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAGATCAGTTCCAGCCAGGTCCAAATTC4230.26220362622036264No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4200.2603440260344026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3940.24422749108941577No Hit
GTTATAGACGGTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAGCGTCACCAACTTCATTTACGTTACC3650.22625135595846893No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAA3650.22625135595846893No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGT3620.2243917557725089No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3610.2237718890438556No Hit
GTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACA3540.2194328219432822No Hit
GGTAAGGGCCCTGGGAAGAGTTATCTTTTCTCCTTGACCGTCTATAACCTTGAAATCGGATTATCCGGAGATAAG3510.21757322175732216No Hit
ATCCTAAGAGATAGGGAAGCTCCGTTTTAAAGTGCATGATTATCCATGCCACCCATCGAAAGGGAATCCGGTTAA3450.21385402138540213No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA3410.2113745544707888No Hit
TCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAGATCAGTTCCAGCCAGGTCCAAATTCTTCCAGCCAA3400.21075468774213543No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3250.20145668681233533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAG3250.20145668681233533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.198977219897722No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3200.19835735316906863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2970.18410041841004182Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.18224081822408184No Hit
CCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC2920.18100108476677515No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2910.18038121803812182Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTTACTTAAGTGAACGTGGGCATGCGAATGAGA2850.17666201766620176No Hit
GTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAATGGATGGCGCTCAAGCGTAGTACCCATACCTCACCG2800.17356268402293507No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT2750.17046335037966837No Hit
GAGTAATACGTTCCGTCGGTTTTTCAAGGACTGTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCC2690.16674415000774834No Hit
GATCAGTTCCAGCCAGGTCCAAATTCTTCCAGCCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTATCCC2640.16364481636448164No Hit
TGATTATCCATGCCACCCATCGAAAGGGAATCCGGTTAAGATTCCGGAACCAGGACATGGATCTTTGACGGTAAC2640.16364481636448164No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2600.1611653494498683No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTAC2590.16054548272121494No Hit
CTTCTGTTGCATCCGGTGCGTCCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTGGTCGTA2580.1599256159925616No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA2530.15682628234929488No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGAGAGTAATACG2500.1549666821633349No Hit
TCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTCTGTTGCATCCG2400.1487680148768015No Hit
GGCTGGAACTGATCTGGGCAACTGGGTTGGATCTGGCCGGGACTGAGTTTGGAAGGCTTAGGCTGCCTTCGGGCG2400.1487680148768015No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACATATATGCTCTTACTCAAATCCATCACTAAAGATCCAGAT2360.14628854796218813No Hit
GAGTACGACCAGGCATGAGAGTAATACGTTCCGTCGGTTTTTCAAGGACTGTTAAGGACGCACCGGATGCAACAG2340.14504881450488144No Hit
GTGCAGCACTTCTGTTGCATCCGGTGCGTCCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCC2330.1444289477762281No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT2310.14318921431892143No Hit
CCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAGATCAGTTCCAGCCAG2250.1394700139470014No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCG2230.1382302804896947No Hit
ACCCATACCTCACCGTCAGCGTTTATGTGACGCGCTGACGAGTAGGCAGGCGTGGAGGTCAGTGAAGAAGCCTTG2220.13761041376104136No Hit
GTGTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAATGGATGGCGCTCAAGCGTAGTACCCATACCTCAC2200.1363706803037347No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGC2140.13265147993181467No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAATGGATGGCGCTCAAGCGTAGTACCCA2140.13265147993181467No Hit
GTGCATGATTATCCATGCCACCCATCGAAAGGGAATCCGGTTAAGATTCCGGAACCAGGACATGGATCTTTGACG2110.13079187974585463No Hit
TCCCAGGGCCCTTACCAGCGTCACCAACTTCATTTACGTTACCGTCAAAGATCCATGTCCTGGTTCCGGAATCTT2100.1301720130172013No Hit
GTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGA2100.1301720130172013No Hit
CTCCTTGACCGTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTC2070.12831241283124128No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGAGAGTAATACGTTCCGTCGGTTTTTCAAGGACTGT2050.1270726793739346No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTT2040.12645281264528127No Hit
CATCAGACACCACAAAAGGTGTTAATTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA2030.12583294591662794No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGC2030.12583294591662794No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG2000.12397334573066791No Hit
GTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAGATC1990.12335347900201457No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT1970.12211374554470789Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG1970.12211374554470789Clontech Universal Primer Mix Long (96% over 25bp)
TGCCTGAATAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCAAATCGATCGTCAA1960.12149387881605456No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAATTAACACCTTTTGTGGTGTCTGAT1920.1190144119014412No Hit
GTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATTTCAAGGTTATAGACGGTCAAGGAGAAA1920.1190144119014412No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC1900.11777467844413451Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1890.11715481171548119Clontech Universal Primer Mix Long (96% over 26bp)
GGTTAAGGATGTTTCATCCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGATGCGAGTTTCTGCTATC1850.1146753448008678No Hit
GTCCCGGCCAGATCCAACCCAGTTGCCCAGATCAGTTCCAGCCAGGTCCAAATTCTTCCAGCCAAGGCCCGATGC1830.11343561134356114No Hit
CAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATTTCAAGGTTATAGACGGTCA1830.11343561134356114No Hit
CCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTCTC1800.11157601115760112No Hit
CTCTTAGGATCGACTAACCCATGTCCAACTGCTGTTTCCATGGAACCTTTCTCCACTTCAGTCTTCAAGGTTCTC1800.11157601115760112No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT1770.1097164109716411No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAACTCGCATCAGTTTTATGAGGTAAAGCGAA1720.1066170773283744No Hit
GCGTAGTACCCATACCTCACCGTCAGCGTTTATGTGACGCGCTGACGAGTAGGCAGGCGTGGAGGTCAGTGAAGA1700.10537734387106772No Hit
TCCCTATCTCTTAGGATCGACTAACCCATGTCCAACTGCTGTTTCCATGGAACCTTTCTCCACTTCAGTCTTCAA1690.10475747714241439No Hit
TTCAAGGACTGTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGA1680.10413761041376103No Hit
GTCTAGATGAATTAACACCTTTTGTGGTGTCTGATGAGCGTAGATTCCGGCACCTTAACCTCACGATCGGTTCAT1650.10227801022780103No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1640.10165814349914767Clontech Universal Primer Mix Long (96% over 26bp)
GTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAGCGTCACCAACTTCATTTACGTTACCGTCAAAGATC1630.10103827677049435No Hit
GGGTTAGTCGATCCTAAGAGATAGGGAAGCTCCGTTTTAAAGTGCATGATTATCCATGCCACCCATCGAAAGGGA1630.10103827677049435No Hit
CTGTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGA1630.10103827677049435No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1630.10103827677049435Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)