FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002147947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161325
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG14310.8870292887029289Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12100.7500387416705409Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6920.428947776228111Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGCCAGA6230.3861769719510305No Hit
GTCCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTC5410.33534790020145666No Hit
GTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATT5320.3297690996435766No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC5270.32666976600030995Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5080.3148922981558965Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCAGCCAGGTCCAAATTCTTCCAGCCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGT5040.3124128312412831No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT4870.30187509685417635No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTTGGGTGCATCG4720.29257709592437625No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGA4630.28699829536649624No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGAGAGTAATACGTTCCGTCGG4600.2851386951805362No Hit
GTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTCTGTTGCATCC4290.26592282659228267No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4220.26158375949170926Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCT3970.24608709127537579No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC3840.2380288238028824Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3730.23121028978769562Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAGATCAGTTCCAGCCAGGTCCAAATTC3700.2293506896017356No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAA3530.21881295521462885No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3520.21819308848597552Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3420.21199442119944212No Hit
GTTATAGACGGTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAGCGTCACCAACTTCATTTACGTTACC3260.2020765535409887No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3250.20145668681233533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTAAGAGATAGGGAAGCTCCGTTTTAAAGTGCATGATTATCCATGCCACCCATCGAAAGGGAATCCGGTTAA3170.19649775298310862No Hit
GTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACA3160.19587788625445532No Hit
GGTAAGGGCCCTGGGAAGAGTTATCTTTTCTCCTTGACCGTCTATAACCTTGAAATCGGATTATCCGGAGATAAG3110.1927785526111886No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGT3100.19215868588253526No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC2870.17790175112350842No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA2800.17356268402293507No Hit
TCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAGATCAGTTCCAGCCAGGTCCAAATTCTTCCAGCCAA2770.17170308383697505No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2700.16736401673640167Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2680.166124283279095Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTTACTTAAGTGAACGTGGGCATGCGAATGAGA2640.16364481636448164No Hit
ATTGTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAG2570.15930574926390828No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT2520.15620641562064155No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT2410.14938788160545483No Hit
CCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC2390.14814814814814814No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2380.1475282814194948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCTGGAACTGATCTGGGCAACTGGGTTGGATCTGGCCGGGACTGAGTTTGGAAGGCTTAGGCTGCCTTCGGGCG2290.14194948086161474No Hit
CTTCTGTTGCATCCGGTGCGTCCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCCTGGTCGTA2260.14008988067565475No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTAC2240.13885014721834807No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGAGAGTAATACG2240.13885014721834807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.13761041376104136No Hit
GTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAATGGATGGCGCTCAAGCGTAGTACCCATACCTCACCG2200.1363706803037347No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2100.1301720130172013No Hit
ACCCATACCTCACCGTCAGCGTTTATGTGACGCGCTGACGAGTAGGCAGGCGTGGAGGTCAGTGAAGAAGCCTTG2090.12955214628854797No Hit
CTCCTTGACCGTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTC2080.1289322795598946No Hit
TCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTCTGTTGCATCCG2060.12769254610258796No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAATGGATGGCGCTCAAGCGTAGTACCCA2010.12459321245932124No Hit
GTGCAGCACTTCTGTTGCATCCGGTGCGTCCTTAACAGTCCTTGAAAAACCGACGGAACGTATTACTCTCATGCC2010.12459321245932124No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA1980.12273361227336121No Hit
GATCAGTTCCAGCCAGGTCCAAATTCTTCCAGCCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTATCCC1950.1208740120874012No Hit
TGATTATCCATGCCACCCATCGAAAGGGAATCCGGTTAAGATTCCGGAACCAGGACATGGATCTTTGACGGTAAC1940.12025414535874787No Hit
GAGTAATACGTTCCGTCGGTTTTTCAAGGACTGTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCC1940.12025414535874787No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1900.11777467844413451Clontech Universal Primer Mix Long (96% over 26bp)
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACATATATGCTCTTACTCAAATCCATCACTAAAGATCCAGAT1900.11777467844413451No Hit
GTGCATGATTATCCATGCCACCCATCGAAAGGGAATCCGGTTAAGATTCCGGAACCAGGACATGGATCTTTGACG1890.11715481171548119No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTT1860.11529521152952116No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCG1780.11033627770029443No Hit
GGTTAAGGATGTTTCATCCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGATGCGAGTTTCTGCTATC1760.10909654424298776No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGC1750.10847667751433443No Hit
GTACACCAGACGCCCGAAGGCAGCCTAAGCCTTCCAAACTCAGTCCCGGCCAGATCCAACCCAGTTGCCCAGATC1730.10723694405702774No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAGC1710.10599721059972106No Hit
GTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATTTCAAGGTTATAGACGGTCAAGGAGAAA1690.10475747714241439No Hit
CATCAGACACCACAAAAGGTGTTAATTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA1650.10227801022780103No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCATGAGAGTAATACGTTCCGTCGGTTTTTCAAGGACTGT1650.10227801022780103No Hit
GGGTTAGTCGATCCTAAGAGATAGGGAAGCTCCGTTTTAAAGTGCATGATTATCCATGCCACCCATCGAAAGGGA1630.10103827677049435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)