FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254857
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG27751.0888459018194516Clontech Universal Primer Mix Long (96% over 26bp)
CGTCTACACTGGGTGGCTGCACACTTACGTGTTCTTGAAAAGACAGCTTCCCCTGTAGACGAGAGCGACTGTGGC24660.967601439238475No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21810.8557740222948554Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGACGCGTCTGTTTGTTTGTTCCCGCCTCCCCCTGGTACGCCGTCGATGTTCGCAGGTTGCAAGAGGGGCA19390.7608188121181682No Hit
CACGTACTCCGTGGAAAAGGGTGCTGACGCACCCTGCTTCATGGTTGTGGGTGAGGAGGGCGCCACAGTCGCTCT15820.6207402582624766No Hit
TCCTGGGACAGCGAATCACCGTGACGTCCCCTAACAGGGACGTCAGTTTCATTGTCGGGCCCGACGTTAAGAAGG12540.49204063455192515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.4630047438367398No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11290.4429935218573553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACGTGTAGGCCTTCTTAACGTCGGGCCCGACAATGAAACTGACGTCCCTGTTAGGGGACGTCACGGTGATT11240.4410316373495725No Hit
TCCCTAGGGCGCCGCGGTTCACCTTCGAACCCGGAGAGAGCTCCCTGCCGCTGGTGGCGAAAGTGACTAACGTCA10920.42847557649976264No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8250.3237109437841613Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7550.29624456067520216Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTACACGTACTCCGTGGAAAAGGGTGCTGACGCACCCTGCTTCATGGTTGTGGGTGAGGAGGGCGCCACAGT7510.2946750530689759No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7380.28957415334874065Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.27230956968025205No Hit
GGCCGCGACCGAACGGCCATAACCAGCATAAGCAACCGCAGAAGCACCTGCACCCATCATCAGCACCAGCACTTT6800.2668162930584602No Hit
GTCGATGACCACGTGCCAAGGAGGCTCGTTACCGCCAAACGACGAAGAGAACAACAAGCCAAGGAGTGAATGCCT6340.24876695558685852No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG6280.24641269417751918Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCAGGACACGCAAGTGTTCTGGGACTCCGTTGAGAACGGAGCCTCGGACCATGACGCTGGTGGATTTCTCCT6260.24562794037440602No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6060.23778040234327485Clontech Universal Primer Mix Long (96% over 26bp)
CACCTGCACCCATCATCAGCACCAGCACTTTCCGTTTTTTGCCATGTCAGGCAAGAAGACTCGTGGTGGAGGCCG5710.22404721078879528No Hit
GCCATAACCAGCATAAGCAACCGCAGAAGCACCTGCACCCATCATCAGCACCAGCACTTTCCGTTTTTTGCCATG5600.21973106487167315No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5570.21855393416700344Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.2169844265607772No Hit
GTCCCAGAACACTTGCGTGTCCTGGGACAGCGAATCACCGTGACGTCCCCTAACAGGGACGTCAGTTTCATTGTC5370.21070639613587228No Hit
CCCTAGGGCGCCGCGGTTCACCTTCGAACCCGGAGAGAGCTCCCTGCCGCTGGTGGCGAAAGTGACTAACGTCAC5290.2075673809234198No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5240.205605496415637Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCCAAGGAGGCTCGTTACCGCCAAACGACGAAGAGAACAACAAGCCAAGGAGTGAATGCCTGGAAGCATCCGT5240.205605496415637No Hit
ATGTAGAGCGGTCCGCCAATACATCGGCTTGTCCAGGAAATGCCGTACGAGCCAAAATCATTGCCAGCTTCGGGC5020.1969732045813927No Hit
TCCTGGGTGTGCCCCTCTTGCAACCTGCGAACATCGACGGCGTACCAGGGGGAGGCGGGAACAAACAAACAGACG4960.19461894317205336No Hit
TTACACACCTAGAAAAGCACTTCCTGGGTGTGCCCCTCTTGCAACCTGCGAACATCGACGGCGTACCAGGGGGAG4810.18873328964870495No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4810.18873328964870495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4780.1875561589440353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGGCCTTCTTAACGTCGGGCCCGACAATGAAACTGACGTCCCTGTTAGGGGACGTCACGGTGATTCGCTGT4710.18480952063313938No Hit
TCCCCTAACAGGGACGTCAGTTTCATTGTCGGGCCCGACGTTAAGAAGGCCTACACGTACTCCGTGGAAAAGGGT4560.17892386710979097No Hit
GCATAAGCAACCGCAGAAGCACCTGCACCCATCATCAGCACCAGCACTTTCCGTTTTTTGCCATGTCAGGCAAGA4420.17343059048799914No Hit
GCCTGGAAGCATCCGTCTCAGTGGCGCAGGAGTAACGGGTGGGCGTGCGGGCACCAGCTCAACCGCGTCCGCGGA4400.17264583668488603No Hit
GGGTGGCTGCACACTTACGTGTTCTTGAAAAGACAGCTTCCCCTGTAGACGAGAGCGACTGTGGCGCCCTCCTCA4380.17186108288177293No Hit
GTTGTGGGTGAGGAGGGCGCCACAGTCGCTCTCGTCTACAGGGGAAGCTGTCTTTTCAAGAACACGTAAGTGTGC4280.16793731386620733No Hit
GTCCGAGGCTCCGTTCTCAACGGAGTCCCAGAACACTTGCGTGTCCTGGGACAGCGAATCACCGTGACGTCCCCT3940.1545964992132843No Hit
TGCCAAGGAGGCTCGTTACCGCCAAACGACGAAGAGAACAACAAGCCAAGGAGTGAATGCCTGGAAGCATCCGTC3880.15224223780394497No Hit
GCTCAACCGCGTCCGCGGAGGGCGAGTACCGGTTGGCCCGCGCGTTACACACCTAGAAAAGCACTTCCTGGGTGT3780.14831846878837934No Hit
GGAGTAACGGGTGGGCGTGCGGGCACCAGCTCAACCGCGTCCGCGGAGGGCGAGTACCGGTTGGCCCGCGCGTTA3760.14753371498526627No Hit
GTACATGGGATAGGCCGCGACCGAACGGCCATAACCAGCATAAGCAACCGCAGAAGCACCTGCACCCATCATCAG3710.14557183047748345No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC3550.1392938000525785Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.1392938000525785No Hit
ACGTTAAGAAGGCCTACACGTACTCCGTGGAAAAGGGTGCTGACGCACCCTGCTTCATGGTTGTGGGTGAGGAGG3490.13693953864323916No Hit
ATGTGTGAGTGAGCCGGGGTCGCACTGGCGAGCCCCCGGTTACCCCGGAGGGCCTCGGGTTTTGCGGGGCACGGA3400.1334081465292301No Hit
AGTAACGGGTGGGCGTGCGGGCACCAGCTCAACCGCGTCCGCGGAGGGCGAGTACCGGTTGGCCCGCGCGTTACA3390.13301576962767356No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3380.132623392726117Clontech Universal Primer Mix Long (96% over 26bp)
GTACCAGGGGGAGGCGGGAACAAACAAACAGACGCGTCTACACTGGGTGGCTGCACACTTACGTGTTCTTGAAAA3380.132623392726117No Hit
CTCCTCACCCACAACCATGAAGCAGGGTGCGTCAGCACCCTTTTCCACGGAGTACGTGTAGGCCTTCTTAACGTC3370.13223101582456045No Hit
AGCCGGGGTCGCACTGGCGAGCCCCCGGTTACCCCGGAGGGCCTCGGGTTTTGCGGGGCACGGAAAGTGTCCTGT3180.12477585469498581No Hit
GGTGAGGAGGGCGCCACAGTCGCTCTCGTCTACAGGGGAAGCTGTCTTTTCAAGAACACGTAAGTGTGCAGCCAC3150.12359872399031614No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3140.12320634708875959Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3070.12045970877786367Clontech SMART CDS Primer II A (96% over 26bp)
ATCAAGGAGAAATCCACCAGCGTCATGGTCCGAGGCTCCGTTCTCAACGGAGTCCCAGAACACTTGCGTGTCCTG2990.1173206935654112No Hit
GTTAGGGGACGTCACGGTGATTCGCTGTCCCAGGACACGCAAGTGTTCTGGGACTCCGTTGAGAACGGAGCCTCG2980.11692831666385464No Hit
CTTCCTGGGTGTGCCCCTCTTGCAACCTGCGAACATCGACGGCGTACCAGGGGGAGGCGGGAACAAACAAACAGA2950.11575118595918495No Hit
GTTCTGGGACTCCGTTGAGAACGGAGCCTCGGACCATGACGCTGGTGGATTTCTCCTTGATAGTGACGTTAGTCA2920.11457405525451528No Hit
GGCGAGTACCGGTTGGCCCGCGCGTTACACACCTAGAAAAGCACTTCCTGGGTGTGCCCCTCTTGCAACCTGCGA2860.11221979384517591No Hit
GTGGTGGAGGCCGCAAATCCTCCAACCGCAGCGTCCCTAGGGCGCCGCGGTTCACCTTCGAACCCGGAGAGAGCT2860.11221979384517591No Hit
GTTCTCAACGGAGTCCCAGAACACTTGCGTGTCCTGGGACAGCGAATCACCGTGACGTCCCCTAACAGGGACGTC2860.11221979384517591No Hit
ACCCACAACCATGAAGCAGGGTGCGTCAGCACCCTTTTCCACGGAGTACGTGTAGGCCTTCTTAACGTCGGGCCC2850.11182741694361936No Hit
CCCGGAGAGAGCTCCCTGCCGCTGGTGGCGAAAGTGACTAACGTCACTATCAAGGAGAAATCCACCAGCGTCATG2840.11143504004206281No Hit
ACACTGGGTGGCTGCACACTTACGTGTTCTTGAAAAGACAGCTTCCCCTGTAGACGAGAGCGACTGTGGCGCCCT2790.10947315553428No Hit
GCCTACACGTACTCCGTGGAAAAGGGTGCTGACGCACCCTGCTTCATGGTTGTGGGTGAGGAGGGCGCCACAGTC2730.10711889412494066No Hit
GCTCTACATCGTGGAAATAGTATACTGTCCTGGCGAACTGGCGAAGCACACCCCTTACTCGCCACCTGAGGCTCC2730.10711889412494066No Hit
TCTTAACGTCGGGCCCGACAATGAAACTGACGTCCCTGTTAGGGGACGTCACGGTGATTCGCTGTCCCAGGACAC2700.10594176342027097No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2690.10554938651871441Clontech Universal Primer Mix Long (96% over 26bp)
CTGTAGACGAGAGCGACTGTGGCGCCCTCCTCACCCACAACCATGAAGCAGGGTGCGTCAGCACCCTTTTCCACG2680.10515700961715786No Hit
CCCCGGAGGGCCTCGGGTTTTGCGGGGCACGGAAAGTGTCCTGTGCCCCTGTCGATGACCACGTGCCAAGGAGGC2670.1047646327156013No Hit
CCCTAACAGGGACGTCAGTTTCATTGTCGGGCCCGACGTTAAGAAGGCCTACACGTACTCCGTGGAAAAGGGTGC2610.10241037130626196No Hit
GCTTCATGGTTGTGGGTGAGGAGGGCGCCACAGTCGCTCTCGTCTACAGGGGAAGCTGTCTTTTCAAGAACACGT2610.10241037130626196No Hit
GCTGTAAGGGTAGTGAGATGTTTAGCGTCACTCGCACTGGCGGGCATGACGTTTGAGGGAACCTCCCTACGGAGG2570.10084086370003571No Hit
GTATTACAGCAGCATGTTGGAAATAACGAAGCTTCTGCATCATAACAAAGGTGCCAAGATAACTGCACTGGTCAA2550.1000561098969226No Hit
CACCCAGGAAGTGCTTTTCTAGGTGTGTAACGCGCGGGCCAACCGGTACTCGCCCTCCGCGGACGCGGTTGAGCT2550.1000561098969226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)