FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254857
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTACACTGGGTGGCTGCACACTTACGTGTTCTTGAAAAGACAGCTTCCCCTGTAGACGAGAGCGACTGTGGC22450.8808861439944754No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19780.7761215112788741Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGACGCGTCTGTTTGTTTGTTCCCGCCTCCCCCTGGTACGCCGTCGATGTTCGCAGGTTGCAAGAGGGGCA17380.6819510549052998No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17220.6756730244803949Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACGTACTCCGTGGAAAAGGGTGCTGACGCACCCTGCTTCATGGTTGTGGGTGAGGAGGGCGCCACAGTCGCTCT12160.4771303122927759No Hit
TCCTGGGACAGCGAATCACCGTGACGTCCCCTAACAGGGACGTCAGTTTCATTGTCGGGCCCGACGTTAAGAAGG10540.4135652542406134No Hit
GAGTACGTGTAGGCCTTCTTAACGTCGGGCCCGACAATGAAACTGACGTCCCTGTTAGGGGACGTCACGGTGATT10420.4088567314219346No Hit
TCCCTAGGGCGCCGCGGTTCACCTTCGAACCCGGAGAGAGCTCCCTGCCGCTGGTGGCGAAAGTGACTAACGTCA9130.3582401111211385No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT8440.33116610491373594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.2573992474211028No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6490.2546526091102069Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6480.25426023220865035Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTACACGTACTCCGTGGAAAAGGGTGCTGACGCACCCTGCTTCATGGTTGTGGGTGAGGAGGGCGCCACAGT6090.2389575330479445No Hit
GTCCCAGGACACGCAAGTGTTCTGGGACTCCGTTGAGAACGGAGCCTCGGACCATGACGCTGGTGGATTTCTCCT5840.22914811050903056No Hit
GGCCGCGACCGAACGGCCATAACCAGCATAAGCAACCGCAGAAGCACCTGCACCCATCATCAGCACCAGCACTTT5470.21463016515143787No Hit
GTCGATGACCACGTGCCAAGGAGGCTCGTTACCGCCAAACGACGAAGAGAACAACAAGCCAAGGAGTGAATGCCT5450.21384541134832477No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5090.19971984289228864Clontech Universal Primer Mix Long (96% over 26bp)
GCCATAACCAGCATAAGCAACCGCAGAAGCACCTGCACCCATCATCAGCACCAGCACTTTCCGTTTTTTGCCATG4870.1910875510580443No Hit
GCCTGGAAGCATCCGTCTCAGTGGCGCAGGAGTAACGGGTGGGCGTGCGGGCACCAGCTCAACCGCGTCCGCGGA4800.1883409127471484No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4750.1863790282393656Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCAAGGAGGCTCGTTACCGCCAAACGACGAAGAGAACAACAAGCCAAGGAGTGAATGCCTGGAAGCATCCGT4580.1797086209129041No Hit
ATGTAGAGCGGTCCGCCAATACATCGGCTTGTCCAGGAAATGCCGTACGAGCCAAAATCATTGCCAGCTTCGGGC4570.17931624401134755No Hit
CACCTGCACCCATCATCAGCACCAGCACTTTCCGTTTTTTGCCATGTCAGGCAAGAAGACTCGTGGTGGAGGCCG4410.17303821358644259No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4370.17146870598021635Clontech Universal Primer Mix Long (96% over 26bp)
TCCTGGGTGTGCCCCTCTTGCAACCTGCGAACATCGACGGCGTACCAGGGGGAGGCGGGAACAAACAAACAGACG4300.16872206766932044No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4210.16519067555531142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCCAGAACACTTGCGTGTCCTGGGACAGCGAATCACCGTGACGTCCCCTAACAGGGACGTCAGTTTCATTGTC4140.1624440372444155No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4100.16087452963818924Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGCCTTCTTAACGTCGGGCCCGACAATGAAACTGACGTCCCTGTTAGGGGACGTCACGGTGATTCGCTGT4080.16008977583507614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.16008977583507614No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3890.15263461470550152Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGGCTGCACACTTACGTGTTCTTGAAAAGACAGCTTCCCCTGTAGACGAGAGCGACTGTGGCGCCCTCCTCA3890.15263461470550152No Hit
TTACACACCTAGAAAAGCACTTCCTGGGTGTGCCCCTCTTGCAACCTGCGAACATCGACGGCGTACCAGGGGGAG3850.15106510709927526No Hit
CCCTAGGGCGCCGCGGTTCACCTTCGAACCCGGAGAGAGCTCCCTGCCGCTGGTGGCGAAAGTGACTAACGTCAC3850.15106510709927526No Hit
GTTGTGGGTGAGGAGGGCGCCACAGTCGCTCTCGTCTACAGGGGAAGCTGTCTTTTCAAGAACACGTAAGTGTGC3850.15106510709927526No Hit
TCCCCTAACAGGGACGTCAGTTTCATTGTCGGGCCCGACGTTAAGAAGGCCTACACGTACTCCGTGGAAAAGGGT3720.14596420737904No Hit
TGCCAAGGAGGCTCGTTACCGCCAAACGACGAAGAGAACAACAAGCCAAGGAGTGAATGCCTGGAAGCATCCGTC3630.142432815265031No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3590.14086330765880473Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATAAGCAACCGCAGAAGCACCTGCACCCATCATCAGCACCAGCACTTTCCGTTTTTTGCCATGTCAGGCAAGA3500.1373319155447957No Hit
GTACATGGGATAGGCCGCGACCGAACGGCCATAACCAGCATAAGCAACCGCAGAAGCACCTGCACCCATCATCAG3320.13026913131677764No Hit
ATGTGTGAGTGAGCCGGGGTCGCACTGGCGAGCCCCCGGTTACCCCGGAGGGCCTCGGGTTTTGCGGGGCACGGA3300.12948437751366454No Hit
GGAGTAACGGGTGGGCGTGCGGGCACCAGCTCAACCGCGTCCGCGGAGGGCGAGTACCGGTTGGCCCGCGCGTTA3260.1279148699074383No Hit
AGCCGGGGTCGCACTGGCGAGCCCCCGGTTACCCCGGAGGGCCTCGGGTTTTGCGGGGCACGGAAAGTGTCCTGT3120.12242159328564647No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3070.12045970877786367Clontech Universal Primer Mix Long (96% over 26bp)
GCTCAACCGCGTCCGCGGAGGGCGAGTACCGGTTGGCCCGCGCGTTACACACCTAGAAAAGCACTTCCTGGGTGT3050.11967495497475056No Hit
GTACCAGGGGGAGGCGGGAACAAACAAACAGACGCGTCTACACTGGGTGGCTGCACACTTACGTGTTCTTGAAAA3010.11810544736852431No Hit
GTCCGAGGCTCCGTTCTCAACGGAGTCCCAGAACACTTGCGTGTCCTGGGACAGCGAATCACCGTGACGTCCCCT2990.1173206935654112No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2960.11614356286074153Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCTGGGTGTGCCCCTCTTGCAACCTGCGAACATCGACGGCGTACCAGGGGGAGGCGGGAACAAACAAACAGA2910.11418167835295873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.11300454764828903No Hit
ACGTTAAGAAGGCCTACACGTACTCCGTGGAAAAGGGTGCTGACGCACCCTGCTTCATGGTTGTGGGTGAGGAGG2820.1106502862389497No Hit
ATCAAGGAGAAATCCACCAGCGTCATGGTCCGAGGCTCCGTTCTCAACGGAGTCCCAGAACACTTGCGTGTCCTG2710.10633414032182753No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2680.10515700961715786Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2620.1028027482078185Clontech Universal Primer Mix Long (96% over 25bp)
GTTCTGGGACTCCGTTGAGAACGGAGCCTCGGACCATGACGCTGGTGGATTTCTCCTTGATAGTGACGTTAGTCA2580.10123324060159226No Hit
GCTTCATGGTTGTGGGTGAGGAGGGCGCCACAGTCGCTCTCGTCTACAGGGGAAGCTGTCTTTTCAAGAACACGT2570.10084086370003571No Hit
GGTGAGGAGGGCGCCACAGTCGCTCTCGTCTACAGGGGAAGCTGTCTTTTCAAGAACACGTAAGTGTGCAGCCAC2550.1000561098969226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)