FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423736
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG99522.3486321671984447Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90132.127031925538543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT43961.0374384050446506Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32980.7783147997809957Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG28650.6761285328600827Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA25130.5930579417373082Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG24200.5711103139690751Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC23220.5479827062133027Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT21050.49677157475409217Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20880.4927596427964582No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC18140.4280967394792984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG17980.4243208035191723Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG17070.402845167745955Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG13280.3134026846904676Clontech Universal Primer Mix Long (96% over 25bp)
GTCCTGGGCAGGTCCATCCTGGCGAACCAAGACAGTCCCATCCTGGTCCCGTGGCTTCCGACTAAGCCCCGACGT13210.3117507127079125No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12810.3023108728075972Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.30042290482753414No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12660.2987709328449789Clontech Universal Primer Mix Long (96% over 26bp)
AACCAAGACAGTCCCATCCTGGTCCCGTGGCTTCCGACTAAGCCCCGACGTGCCCAAGCCTTAGAGCCAATCCTT12360.2916910529197425No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT10550.24897577737081578Clontech SMART CDS Primer II A (96% over 26bp)
GTCCCATCCTGGTCCCGTGGCTTCCGACTAAGCCCCGACGTGCCCAAGCCTTAGAGCCAATCCTTTTCCCGAAGT10480.24732380538826063No Hit
GAGCTAGGGTTCAATGGCTGGTAGAGCTCGGCACTTTTGCCGGGTCCGGTGCGTTCCTGACAGTCCTTGAAAATC9650.2277361375951064No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9140.2157003417222044Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8680.20484452583684182Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG8610.20319255385428664Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.2008325938792078No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG8450.19941661789416054Clontech Universal Primer Mix Long (96% over 26bp)
GTCTACCACCCTGAAATCGTATTGTACGGAGCTAGGGTTCAATGGCTGGTAGAGCTCGGCACTTTTGCCGGGTCC8450.19941661789416054No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC8430.19894462589914474Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGCTTGTTAAGCGCACGCCGGATGCCCGAAAGCAGACAAAGCCGTCCACCGACAAGTCCTGGGCAG7970.18808881001378217No Hit
GTACATGGGTTTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA7860.18549285404119545No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7660.1807729340910378Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7230.17062510619819887Clontech Universal Primer Mix Long (96% over 26bp)
CCCTAGCTCCGTACAATACGATTTCAGGGTGGTAGACTGTTAAGGAGAAAAGAAAACTCTTCCCGGGGTCCCTGC6890.16260124228293088No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT6680.15764532633526535Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA6600.1557573583552023Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.15528536636018653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6520.15386939037513925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTAGACTGTTAAGGAGAAAAGAAAACTCTTCCCGGGGTCCCTGCCAGCGTCTCCAAGTTCATTTACGTTGCC6400.15103743840504466No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT6190.14608152245737913Clontech Universal Primer Mix Long (96% over 25bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGTGTGA6130.14466554647233182No Hit
GTTCCTGACAGTCCTTGAAAATCCAAGGGAATGAATAATTTTCACACCTGGTCGTACTCATAACCGCAGCAGGTC6080.1434855664847924No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCTTTAGATGGAATTTACCACCCATTTTGAGCTGCATTCC6080.1434855664847924No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGTGTGAAAATTATTCATTCCCTTGG5840.13782166254460324No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5660.13357373458946137Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA5480.12932580663431947Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCAGGCCGTCCGAGTTGTAATCTAGAGAGGTGTTTTCCGTGCCGGACCGTGTACAAGTCCCTTGGAACAGGG5420.1279098306492722No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5310.12531387467668548Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGAGCTAGGGTTCAATGGCTGGTAGAGCTCGGCACTTTTGCCGGGTCCGGTGCGTTCCTGACAGTCCTTGA5300.1250778786791776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT5270.12436989068665394Clontech SMART CDS Primer II A (100% over 26bp)
GTCAGGAACGCACCGGACCCGGCAAAAGTGCCGAGCTCTACCAGCCATTGAACCCTAGCTCCGTACAATACGATT5150.12153793871655935No Hit
ATCCTAAGAGATAGGGAAGCTCCGTTTTAAAGTACGCGATTTTTCGCGTCGCCTATCGAAAGGGAATCCAGTTAA5100.12035795872901996No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGCTTGGGCACGTCG5100.12035795872901996No Hit
AGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAAC4920.11611003077387809No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA4570.1078501708611022Clontech SMART CDS Primer II A (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA4460.1052542148885155Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT4410.10407423490097609No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC4330.10218626692091302Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT4300.10147827892838937No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.10077029093586573No Hit
GTGTATCACAGAGCACCGGGAGTCCGTGTCATGGACGGGATTCTCACCCTCTATGACGCCCTGTTCCAAGGGACT4260.10053429493835785No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT4240.10006230294334208Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)