FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002147971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423736
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG71101.6779315422810428Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG67051.5823531632903505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT32870.7757188438084089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC26760.6315252893310929Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22090.521315158494912Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA18810.4439084713123266Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG18180.42904072346933Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC17710.41794891158645947Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT15550.3669737761247569Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG14550.3433741763739687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC13740.3242585005758302Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG13150.31033473672286516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG10270.24236788944059512Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.2409519134555478No Hit
GTCCTGGGCAGGTCCATCCTGGCGAACCAAGACAGTCCCATCCTGGTCCCGTGGCTTCCGACTAAGCCCCGACGT10000.23599599750788228No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9840.23222006154775618Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCATCCTGGTCCCGTGGCTTCCGACTAAGCCCCGACGTGCCCAAGCCTTAGAGCCAATCCTTTTCCCGAAGT9250.2182962976947911No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC9240.21806030169728322Clontech Universal Primer Mix Long (96% over 26bp)
AACCAAGACAGTCCCATCCTGGTCCCGTGGCTTCCGACTAAGCCCCGACGTGCCCAAGCCTTAGAGCCAATCCTT9220.21758830970226745No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT8690.20508052183434972Clontech SMART CDS Primer II A (96% over 26bp)
GAGCTAGGGTTCAATGGCTGGTAGAGCTCGGCACTTTTGCCGGGTCCGGTGCGTTCCTGACAGTCCTTGAAAATC8250.19469669794400288No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7620.17982895010100627Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTTTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA7580.17888496611097476No Hit
GTCTACCACCCTGAAATCGTATTGTACGGAGCTAGGGTTCAATGGCTGGTAGAGCTCGGCACTTTTGCCGGGTCC7280.17180508618573828No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG6910.16307323427794665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6890.16260124228293088Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6590.15552136235769443Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC6440.15198142239507617Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGCTTGTTAAGCGCACGCCGGATGCCCGAAAGCAGACAAAGCCGTCCACCGACAAGTCCTGGGCAG6220.1467895104499028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.13994562652217418No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5750.13569769856703232Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5660.13357373458946137Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCTTTAGATGGAATTTACCACCCATTTTGAGCTGCATTCC5650.13333773859195347No Hit
GTTCCTGACAGTCCTTGAAAATCCAAGGGAATGAATAATTTTCACACCTGGTCGTACTCATAACCGCAGCAGGTC5440.12838182264428796No Hit
GTCTCAGGCCGTCCGAGTTGTAATCTAGAGAGGTGTTTTCCGTGCCGGACCGTGTACAAGTCCCTTGGAACAGGG5260.12413389468914607No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGTGTGA5230.12342590669662241No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG5220.12318991069911454Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT5180.122245926709083Clontech Universal Primer Mix Long (96% over 26bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGTGTGAAAATTATTCATTCCCTTGG5060.11941397473898842No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGCTTGGGCACGTCG4980.11752600675892537No Hit
GTGGTAGACTGTTAAGGAGAAAAGAAAACTCTTCCCGGGGTCCCTGCCAGCGTCTCCAAGTTCATTTACGTTGCC4830.11398606679630713No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT4820.11375007079879926Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4790.1130420828062756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAAC4780.11280608680876772No Hit
CCCTAGCTCCGTACAATACGATTTCAGGGTGGTAGACTGTTAAGGAGAAAAGAAAACTCTTCCCGGGGTCCCTGC4770.11257009081125985No Hit
GTACGGAGCTAGGGTTCAATGGCTGGTAGAGCTCGGCACTTTTGCCGGGTCCGGTGCGTTCCTGACAGTCCTTGA4730.11162610682122831No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT4700.11091811882870467No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG4560.1076141748635943Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA4500.10619819887854702Clontech Universal Primer Mix Long (96% over 26bp)
GTGTACAAGTCCCTTGGAACAGGGCGTCATAGAGGGTGAGAATCCCGTCCATGACACGGACTCCCGGTGCTCTGT4410.10407423490097609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.1012422829308815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)