FastQCFastQC Report
Wed 8 May 2024
EGAF00002147997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002147997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227312
Total Bases17 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC35

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA54122.380868585908355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35541.5634898289575563No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34831.5322552262968958No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32401.4253536988808335No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA24241.0663757302738088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.8596114591398606No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA19240.8464137397057788No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA17470.7685471950446963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.6695642992890829No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14690.6462483282888717No Hit
ATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14380.6326106848736538No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA13340.5868585908355036No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11380.5006334905328359No Hit
CGATTAAGCTGCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTTC8620.37921447173928347No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8250.36293728443724926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.36293728443724926No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7980.35105933694657565No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA7520.3308228338143169No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6700.29474906736115997No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5810.2555958330400507No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC5380.2366791018512001No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA5320.23403955796438375No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5030.22128176251143802No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA5030.22128176251143802No Hit
TCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.2151228267755332No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.2028049553037235No Hit
GGAATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.18300837615260085No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4110.18080875624692053No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA3980.17508974449215176No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.17464982051101569No Hit
GTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3710.16321179700147814No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA3690.16233194903920603No Hit
CAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.16013232913352574No Hit
ACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA3610.15881255719011755No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA3570.1570528612655733No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA3500.1539733933976209No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC3310.14561483775603576No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA3270.1438551418314915No Hit
CAGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3190.14033574998240306No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.1385760540578588No Hit
TATCAACGCAGAGAACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.1341768142464982No Hit
AACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.13285704230309003No Hit
GTATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAA3000.1319771943408179No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAA2940.12933765045400153No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.12801787851059337No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAA2840.12493841064264094No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2840.12493841064264094No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA2790.12273879073696065No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2750.12097909481241641No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2730.12009924685014431No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAG2720.11965932286900824No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.11789962694446401No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.11613993101991975No Hit
ACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2570.11306046315196734No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.11174069120855916No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAA2500.10998099528401492No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC2450.10778137537833463No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAC2310.1016224396424298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)