FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382160
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22260.5824785430186309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.30484613774335356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.23236340799665062No Hit
CGATTAAGCTGCTGTCTCTTATACACATCTGACGCTGCCGACGACAGTACTCGTGTAGATCTCGGTGGTCGCCGT6610.17296420347498428Illumina RNA PCR Primer (100% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.12115344358383923No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)