FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247188
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56712.2942052203181387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40081.6214379338802856No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22430.907406508406557TruSeq Adapter, Index 2 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.7828049905335209No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.7067495185850446No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.4437917698270143No Hit
CAGCTTAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCT9570.38715471624836156TruSeq Adapter, Index 2 (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.3450814764470767No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.32768580999077623No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.317167500040455No Hit
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6000.24273022962279725TruSeq Adapter, Index 2 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3140.12702882016926387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2840.11489230868812401No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2590.10477854912050748RNA PCR Primer, Index 32 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)